Heterosis studies and molecular characterization of three-line rice hybrids
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Published
Mar 2, 2024
Ravi Kumar
Ravi Kant
M. Vennela
D. Dinesh Varma
https://orcid.org/0000-0002-8640-4262
D. Dinesh Varma
Abstract
The present investigation was undertaken at the Hybrid Rice plot of TCA, Dholi, and Muzaffarpur, and molecular analysis was conducted in the Molecular Laboratory of Postgraduate Dept. of Genetics & Plant Breeding, RPCAU, and Bihar to generate heterosis studies for 18 traits and molecular characterization using SSR markers. The experimental material comprised 31 three-line rice hybrids and 3 commercial checks evaluated in the RBD design. Among the tested varieties, Rajendra Sweta performed best in terms of grain yield per plant. Two rice hybrid genotypes, namely, IR68897A × KMR-3R and Rajendra-3A × RRR–4, exhibited superior standard heterosis over all three tests for trait grain yield per plant. By utilizing 12 primer pairs, a total of 33 shared alleles and 13 unique alleles were produced as amplified products. Among the 12 primers, seven primers were found to be comparatively informative for all nineteen hybrids and eleven parents. Only five primers, namely, MRG2894, RM515, RM520, RM538, and RM555, were able to confirm the hybridity (F1) with the respective parental lines.
How to Cite
Kumar, R., Kant, R., Vennela, M., & Varma, D. D. (2024). Heterosis studies and molecular characterization of three-line rice hybrids. Environment Conservation Journal. https://doi.org/10.36953/ECJ.25982744
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Keywords
Grain yield, Hybrid, Molecular marker, Rice (Oryza sativa), Standard heterosis
References
Aananthi N., & Jebaraj, S. (2023). Heterosis in two line rice hybrids. Indian Journal of Agricultural Research, 40 (3): 178-183.
Akter, M. B., Mosab-Bin, A., Kamruzzaman, M., Reflinur, R., Nahar, N., Rana, M. S., Hoque M. I., & Islam M. S. (2022). Morpho-molecular diversity study of rice cultivars in Bangladesh. Czech Journal of Genetics and Plant Breeding, 58: 64−72.
Anderson, J. A., Chhurchill, G. A., Autrique, J. E., Tanksley, S. D., & Sorrells, M. E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36: 181-186.
Azad, A. K., Sarker, U., Ercisli, S., Assouguem, A., Ullah, R., Almeer, R., Sayed, A. A., & Peluso, I. (2022). Evaluation of Combining Ability and Heterosis of Popular Restorer and Male Sterile Lines for the Development of Superior Rice Hybrids. Agronomy, 12: 965.
Brar, D. S. and Khush, G. S. (2006). Cytogenetic manipulation and germplasm enhancement of rice (Oryza sativa L.). Genetic resources, chromosome engineering and crop improvement, 2: 115-158.
Deepika, C., Devaraju, P. J., Patted, V. S., Niranjan, K., & Kumar, H. D. M. (2022). Molecular Characterization of Land Races of Rice by Using SSR Markers (Oryza sativa L.). International Journal of Environment and Climate Change, 12 (11): 3201-3210.
Kumar, C. P. S., Sathiyabama, R., Suji, D. B., & Muraleedharan, A. (2020). Estimation of heterosis for earliness and certain growth characters in rice (Oryza sativa L.). Plant Archives, 20(2): 1429-1432.
Liu, G. F., Yang, J. X. H. M., Hayat, Y., & Zhu, J. (2008). Genetic analysis of grain yield conditioned on its component traits in rice (Oryza sativa L.). Australian Journal of Agricultural Research, 59 (2): 189-195.
Meredith, W. R., & Bridge, R. R. (1972). Heterosis and gene action in cotton. Crop Science, 12:304-310.
Muthayya, S., Sugimoto, J. D., Montgomery, S., & Maberly, G. F. (2014). An overview of global rice production, supply, trade, and consumption. Annals of the new york Academy of Sciences, 1324 (1): 7-14.
Ramakrishna, T., Krishna, L., Chandra mohan, Y., Gouri Shankar, V., & Saida Naik, D. (2023). Heterosis studies for grain yield and yield attributes in rice Oryza sativa. L hybrids. Electronic journal of Plant Breeding, 14 (1): 279-295.
Ranjith, R. R. S., Saravanan, K. R., Karthikeyan, P., Anbananthan, V., Sathiyanarayanan, G., & Amarnath, T. (2020). Studies on heterosis breeding for qualitative and quantitative traits in rice (Oryza sativa L.). Plant Archives, 20: 1349-1353.
Rohlf, F. J. (2000). NTSYS pc: Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Publications, New York, USA.
Saravanan, K. R., Yogini, D., Vinothkumar, K., & Prakash, M. (2018). Heterosis and combining ability analysis for yield and quality traits in rice (Oryza sativa. L). Pharmacognogy and Phytochemistry, 7 (1S): 73-74.
Shukla, D. K., Singh, S. N., Gaur, S. C., & Anil, K. (2020). Effects of heterosis for yield and yield contributing characters in rice (Oryza sativa L.) under Sodic Soil. Current Journal of Applied Science and Technology, 39 (12): 53-63.
Singh, B., Singh, S. P., & Kumar, J. (2011). Assessment of genetic diversity of aromatic rices (Oryza sativa L.) using morphological, physiochemical and SSR markers. Indian Journal of Genetics and Plant Breeding, 71 (3): 214-222.
Singh, N., Choudhury, D. R., Tiwari, G., Singh, A. K., Kumar, S., Srinivasan, K., Tyagi, R. K., Sharma, A. D., Singh, N. K., & Singh, R. (2016). Genetic diversity trend in Indian rice varieties: an analysis using SSR markers. BMC Genetics, 17 (1):127.
Sreelakshmi, Ch., Ramesh Babu, P., Reddy, K. H. P., Anil Kumar, P., & Latha, P. (2019). Heterosis for quality traits in rice (Oryza sativa L.). Electronic Journal of Plant Breeding, 10 (4): 1586-1592.
Thete A. M., Dahat D. V., Jambhale V. M., & Chaudhari S. R. (2023). Molecular Characterization of Rice Genotypes Using Molecular Markers. International Journal of Plant & Soil Science, 35(20): 284-301.
Toppo, A., Rastogi, N. K., & Sarawgi, A. K. (2018). Characterization of Rice Accessions Using Microsatellite Markers. Indian Journal of Plant Genetic Resources, 31 (3): 310-314.
Vabna, F. A., Islam, M. Z., Prince, Md. F. R. K., & Hoque, Md. E. (2021). Molecular Diversity Analysis in Boro Rice (Oryza sativa L.) Landraces using SSR Markers. Asian Journal of Biology, 12 (1): 36-48.
Yan, W., Hunt, L. A., Johnson, P., Stewart, G., & Lu, X. (2002). On-farm strip trials vs replicated performance trials for cultivar evaluation. Crop Science, 42 (2): 385.
Zhang, S., Huang, X., & Han, B. (2021). Understanding the genetic basis of rice heterosis: Advances and prospects. The Crop Journal, 9 (3): 688-692.
Akter, M. B., Mosab-Bin, A., Kamruzzaman, M., Reflinur, R., Nahar, N., Rana, M. S., Hoque M. I., & Islam M. S. (2022). Morpho-molecular diversity study of rice cultivars in Bangladesh. Czech Journal of Genetics and Plant Breeding, 58: 64−72.
Anderson, J. A., Chhurchill, G. A., Autrique, J. E., Tanksley, S. D., & Sorrells, M. E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36: 181-186.
Azad, A. K., Sarker, U., Ercisli, S., Assouguem, A., Ullah, R., Almeer, R., Sayed, A. A., & Peluso, I. (2022). Evaluation of Combining Ability and Heterosis of Popular Restorer and Male Sterile Lines for the Development of Superior Rice Hybrids. Agronomy, 12: 965.
Brar, D. S. and Khush, G. S. (2006). Cytogenetic manipulation and germplasm enhancement of rice (Oryza sativa L.). Genetic resources, chromosome engineering and crop improvement, 2: 115-158.
Deepika, C., Devaraju, P. J., Patted, V. S., Niranjan, K., & Kumar, H. D. M. (2022). Molecular Characterization of Land Races of Rice by Using SSR Markers (Oryza sativa L.). International Journal of Environment and Climate Change, 12 (11): 3201-3210.
Kumar, C. P. S., Sathiyabama, R., Suji, D. B., & Muraleedharan, A. (2020). Estimation of heterosis for earliness and certain growth characters in rice (Oryza sativa L.). Plant Archives, 20(2): 1429-1432.
Liu, G. F., Yang, J. X. H. M., Hayat, Y., & Zhu, J. (2008). Genetic analysis of grain yield conditioned on its component traits in rice (Oryza sativa L.). Australian Journal of Agricultural Research, 59 (2): 189-195.
Meredith, W. R., & Bridge, R. R. (1972). Heterosis and gene action in cotton. Crop Science, 12:304-310.
Muthayya, S., Sugimoto, J. D., Montgomery, S., & Maberly, G. F. (2014). An overview of global rice production, supply, trade, and consumption. Annals of the new york Academy of Sciences, 1324 (1): 7-14.
Ramakrishna, T., Krishna, L., Chandra mohan, Y., Gouri Shankar, V., & Saida Naik, D. (2023). Heterosis studies for grain yield and yield attributes in rice Oryza sativa. L hybrids. Electronic journal of Plant Breeding, 14 (1): 279-295.
Ranjith, R. R. S., Saravanan, K. R., Karthikeyan, P., Anbananthan, V., Sathiyanarayanan, G., & Amarnath, T. (2020). Studies on heterosis breeding for qualitative and quantitative traits in rice (Oryza sativa L.). Plant Archives, 20: 1349-1353.
Rohlf, F. J. (2000). NTSYS pc: Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Publications, New York, USA.
Saravanan, K. R., Yogini, D., Vinothkumar, K., & Prakash, M. (2018). Heterosis and combining ability analysis for yield and quality traits in rice (Oryza sativa. L). Pharmacognogy and Phytochemistry, 7 (1S): 73-74.
Shukla, D. K., Singh, S. N., Gaur, S. C., & Anil, K. (2020). Effects of heterosis for yield and yield contributing characters in rice (Oryza sativa L.) under Sodic Soil. Current Journal of Applied Science and Technology, 39 (12): 53-63.
Singh, B., Singh, S. P., & Kumar, J. (2011). Assessment of genetic diversity of aromatic rices (Oryza sativa L.) using morphological, physiochemical and SSR markers. Indian Journal of Genetics and Plant Breeding, 71 (3): 214-222.
Singh, N., Choudhury, D. R., Tiwari, G., Singh, A. K., Kumar, S., Srinivasan, K., Tyagi, R. K., Sharma, A. D., Singh, N. K., & Singh, R. (2016). Genetic diversity trend in Indian rice varieties: an analysis using SSR markers. BMC Genetics, 17 (1):127.
Sreelakshmi, Ch., Ramesh Babu, P., Reddy, K. H. P., Anil Kumar, P., & Latha, P. (2019). Heterosis for quality traits in rice (Oryza sativa L.). Electronic Journal of Plant Breeding, 10 (4): 1586-1592.
Thete A. M., Dahat D. V., Jambhale V. M., & Chaudhari S. R. (2023). Molecular Characterization of Rice Genotypes Using Molecular Markers. International Journal of Plant & Soil Science, 35(20): 284-301.
Toppo, A., Rastogi, N. K., & Sarawgi, A. K. (2018). Characterization of Rice Accessions Using Microsatellite Markers. Indian Journal of Plant Genetic Resources, 31 (3): 310-314.
Vabna, F. A., Islam, M. Z., Prince, Md. F. R. K., & Hoque, Md. E. (2021). Molecular Diversity Analysis in Boro Rice (Oryza sativa L.) Landraces using SSR Markers. Asian Journal of Biology, 12 (1): 36-48.
Yan, W., Hunt, L. A., Johnson, P., Stewart, G., & Lu, X. (2002). On-farm strip trials vs replicated performance trials for cultivar evaluation. Crop Science, 42 (2): 385.
Zhang, S., Huang, X., & Han, B. (2021). Understanding the genetic basis of rice heterosis: Advances and prospects. The Crop Journal, 9 (3): 688-692.
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