Morpho-physiological and biochemical attributes as tools to screen tolerance and susceptible rice cultivars for drought stress
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https://orcid.org/0009-0008-8043-2503
Jitendra J. Dhruv
Suresh M. Bambhaneeya
Abstract
Present research work was aimed to observe possible changes in the metabolism of rice plants (Oryza sativa L.) through drought stress. Rice belongs to the family Poaceae. It is considered as a vital food crop across all the major countries worldwide. Rice is prone to be affected by drought stress. Therefore, developing the drought tolerant cultivars of cereal crops assumed considerable importance. This work was carried out with an objective to study the Screening of rice cultivars against water stress and compare biochemical characteristic among different drought tolerant and sensitive rice cultivars. A set of 25 cultivars of rice were screened against drought stress at vegetative stage through various morpho-physiological characters such as moisture, relative water content (RWC), membrane stability index(MSI), membrane injury(MI), seedling length and seedling weight. The RWC is a best criterion for plant water status. The osmotic adjustment is a influential mechanism of conserving cellular hydration under water stress and RWC expression also affects osmotic adjustment in this respect. Thus, it can be considered that the higher RWC having cv. GAR-13 and NWGR-16026 were tolerant and lower RWC having cv. NWGR-16009 and NWGR-16019 were susceptible. Hence, cv. GAR-13 & NWGR-16026 was used as tolerant and NWGR-16009 & NWGR-16019 were used as susceptible. On the basis of first experiment total four cultivars (Two tolerant NWGR-16026 & GAR-13, two susceptible NWGR-16009 & NWGR-16019) were selected for various biochemical analysis. The results indicated that total soluble sugars (TSS), glycine betaine and ascorbic acid content were found significantly higher in cultivar NWGR-16026. The proline content was found significantly higher in cultivar GAR-13. So, RWC and some biochemical parameters are best indicators for selection regarded as potentially useful for drought tolerant rice cultivars and targets for development through transgenic approaches.
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Ascorbic acid, Drought, Glycine betaine, Proline, Rice, RWC, Total soluble sugars
Al-Ashkar, I. M., Zaazaa, E. I., El Sabagh, A. &Barutçular, C. (2016). Physio-biochemical and molecular characterization for drought tolerance in rice genotypes at early seedling stage. Journal of Experimental Biology and Agricultural Sciences, 4(6): 675-687.
Aly, A. A. & Latif, H. H. (2011). Differential effects of paclobutrazol on water stress alleviation through electrolyte leakage, phytohormones, reduced glutathione and lipid peroxidation in some wheat genotypes (Triticumaestivum L.) grown in-vitro.Romanian Biotechnology Letters, 16(6): 6710-721.
Anonymous, (2018-19). United States Department of Agriculture.
Anthon, G and Barrett, D. (2003). Modified method for the determination of pyruvic acid with dinitrophenylhydrazine. In the assessment of onion pungency. J. of the Science of Food and Agriculture. 83(12):1210 - 1213
Atarzyna, K. S. Eidler, Kowska, Hanna, B, Andurska, Jan, B. Ocianowsk. (2010). Evaluation of Cell Membrane Injury In Caraway (Carum Carvi L.) Genotypes In Water Deficit Conditions. Acta Societatis Botanicorum Polonia, 79 (2):95-99.
Bhushan, D., Pandey, A., Choudhary, M. K., Datta, A., Chakraborty, S. & Chakraborty, N. (2007). Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Molecular & Cellular Proteomics, 6(11): 1868-1884.
Chaves, M. M. & Oliveira, M. M. (2004). Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany, 55: 2365-2384.
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. & Basra, S. M. A. (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212.
Garg, B., Jaiswal, J. P., Misra, S., Tripathi, B. N. & Prasad, M. (2012). A comprehensive study on dehydration-induced antioxidative responses during germination of Indian bread wheat cultivars collected from different agroclimatic zones. Physiology and Molecular Biology of Plants, 18(3): 217- 228.
Hameed, A., Goher, M. & Iqbal, N. (2013). Drought induced programmed cell death and associated changes in antioxidants, proteases and lipid peroxidation in wheat. BiologiaPlantarum, 57(2): 370-374.
Hansen, J. M., Go, Y. M. & Jones, D. P. (2006). Nuclear and mitochondrial compartmentation of oxidative stress and redox signalling. Annual Review Pharmacology Toxicology, 46: 215-34.
Hasheminasab, H., Assad, M. T., Aliakbari, A. &Sahhafi, S. R. (2012). Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. Journal of Agricultural Sciences, 4(8), 20-30.
Jayaweera, J.K.P.T.P., Herath, H.M.V.G., Jayatilake, D.V., Udumulla, G.S. & Wickramasinghe, H.A.M. (2016). Physiological, biochemical and proteomic responses of Rice (Oryza sativa L.) varieties Godaheenati and Pokkali for drought stress at the seedling stage. Tropical Agricultural Research, 27(2): 159-170.
Khattab, H. I., Emam, M. A., Emam, M. M., Helal, N. M. & Mohamed, M. R. (2014). Effect of selenium and silicon on transcription factors NAC5 and DREB2A involved in drought-responsive gene expression in rice. Biologia Plantarum, 58 (2): 265-273.
Khush, G. S. (2005). What will it take to feed 5.0 billion rice consumers in 2030.Plant Molecular Biology, 59: 1-6.
Kumari, A. & Sairam, R. K. (2013). Moisture stress induced increases in the activity of enzymes of osmolytes biosynthesis are associated with stress tolerance in wheat genotypes. Indian Journal of Plant Physiology, 18(3): 223-230.
Kumari, R., Choudhury, D., Goswami, S. & Dey, N. (2019). Physiological, biochemical and molecular screening of selected upland rice (Oryza sativa L.) lines from eastern India. Bulletin of the National Research Centre, 43(1): 56.
Mahajan, S. & Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Archives of biochemistry and biophysics, 444: 139-158.
Mostajeran, A. & Rahimi-Eichi, V. (2009). Effects of drought stress on growth and yield of rice (Oryza sativa L.) cultivars and accumulation of proline and soluble sugars in sheath and blades of their different ages leaves. Agriculture & Environment Science, 5(2): 264-272.
Noorka, I. R. & Silva, J. A. T. (2012). Mechanistic insight of water stress induced aggregation in wheat (Triticumaestivum L.) quality: The Protein paradigm shift. Notulae Scientia Biologicae, 4(4): 32-38.
Reddy, A. R., Chaitanya, K. V. & Vivekanandan, M. (2004). Drought induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161: 1189-1202.
Shamsul, H., Qaiser, H., Alyemeni, M. N., Wani, A. S., Pichtel, J. & Aqil, A. (2012). Role of proline under changing environments: a review. Plant Signalling and Behaviour, 7(11): 1456-1466.
Sokoto, M. B. & Muhammad, A. (2014). Response of rice varieties to water stress in sokoto, sudan savannah, nigeria. Journal of Biosciences and Medicines, 2: 68-74.
Surapornpiboon, P., Julsrigival, S., Senthong, C. & Karladee, D. (2008). Effects of silicon on upland rice under drought condition. Chiang Mai University Journal of Natural Sciences, 7(1): 163-171.
Verslues, P. E., Agarwal, M., Katiyar-Agarwal, S., Zhu, J. & Zhu, J. K. (2006).Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. The Plant Journal, 45: 523-539.
Zain, N. A., Ismail, M. R., Puteh, A., Mahmood, M. & Islam, M. R. (2014).Impact of cyclic water stress on growth, physiological responses and yield of rice (Oryza sativa L.) grown in tropical environment. Ciencia Rural, 44(12): 2136-2141.
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