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Abstract
A primary cell culture has been established from the Snow trout Schizothorax plagiostomus. Primary cultures have developed from caudal fin tissues by explant culture technique. Cell outgrowth has been obtained from the caudal fin explant after 5-7 days of explant culture. The culture medium used for the experiment was Leibovitz-15 supplemented with 20% Fetal Bovine Serum. Radiation of cells from the explants started after 2 to 3 days. Both fibroblast like and epithelial like cells were noticed but fibroblast like cells dominated as the growth progressed.
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References
- Chen, S.L., Sha Z. X., and Ye, H.Q. 2003a. Establishment of a pluripotent embryonic cell line from sea perch blastula embryo. Aquaculture 218:141-151.
- Chen, S.L., Ren, G.C., Sha, Z.X. and Shi, C.Y. 2004. Establishment of a continuous embryonic line from flounder Paralichthys olivaceus for virus isolation. Dis. Aquat. Org. 60: 241-246
- Chen, S.L., Ren, G.C., Sha, Z.X. and Hong, Y. 2005. Development and characterization of a continuous embryonic cell line from turbot Scophthalmus maximus. Aquaculture 249: 63-68.
- Chi, S.C., Hu, W.W. and Lo, B.J. 1999. Establishment and characterization of a continuous cell line (GF-1) derivered from grouper Ephinephelus coioides (Hamilton): a cell line susceptible to grouper nervous necrosis virus (GNNV). J. Fish Dis. 22:173-182.
- Fryer, J.L. and Lannan, C.N. 1994. Three decades of fish cell culture: a current listing of cell lines derived from fishes. Journal of Tissue Culture Methods 10:57-94.
- Goswami, M., Sharma, B.S., Tripathi, A.K., Yadav, K., Bahuguna, S.N., Nagpure, N.S., Lakra, W.S., Jena, J.K. 2012. Development and characterization of cell culture systems from Puntius (Tor) chelynoides (McClelland). Gene 500(1): 140-147.
- Hameed, A.S. Sahul, Parameswaran, V., Shukla, R., Singh, I.S. Bright, Thirunavukkarasu, A.R. and Bhonde, R.R. 2006. Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass Lates calcarifer. Aquaculture 257 (1-4): 92-103.
- Kumar, G.S., I.B.S. Singh and R. Philip 1998. Efficacy of fish and prawn muscle extracts as supplements of medium in development of primary cell culture system from larval tissue of aquarium fish, Poecilia reticulata. Indian J. Exp. Biol. 36: 91-94.
- Kumar, G.S., I.B.S. Singh and R. Philip 2001. Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus). Aquaculture 194: 51-62.
- Lakra, W.S., Bhonde, R.R. 1996. Development of primary cell culture from the caudal fin of an Indian major carp, Labeo rohita (Ham.). Asian Fish Sci. 9:149-152.
- Lakra, W.S., Bhonde, R.R., Sivakumar, N., Ayyappan, S. 2006. A new fibroblast like cell line from the fry of golden mahseer Tor putitora (Ham). Aquaculture 253(1-4): 238-243.
- Lakra, W.S., Sivakumar, N., Goswami, M., Bhonde, R.R. 2006. Development of two cell culture systems from Asian seabass Lates calcerifer (Bloch). Aquacult Res. 37:18-24.
- Rao, K.S., Joseph, M.A., Shanker, K.M., Mohan, C.V. 1997. Primary cell culture from explants of heart tissue of Indian Major Carps. Curr. Sci. 73: 374-375.
- Singh, N., Bahuguna S.N., Bhatt, K.C. 1993. The profile of river ecosystem, food and feeding habits of Hill-stream fishes and consequences of recent environmental degradation in garhwal Himalaya. Acta Ichthylologica,Vol.XXIII.
- Sathe, P.S., Maurya, D.T., Basu, A., Gogate, S.S., Banerjee, K. 1995. Establishment and characterization of a new fish cell line, MG-3, from the gills of Mrigal Cirrhinus mrigala. Indian J. Exp. Biol. 33: 589-594.
- Sathe, P.S., Basu, A., Mourya, D.T., Marathe, B.A., Gogate, S.S. and Banerjee, K. 1997. A cell line from the gill tissues of Indian cyprinoid, Labeo rohita. In Vitro Cell Dev. Biol. Anim. 33 (6): 425-427.
References
Chen, S.L., Sha Z. X., and Ye, H.Q. 2003a. Establishment of a pluripotent embryonic cell line from sea perch blastula embryo. Aquaculture 218:141-151.
Chen, S.L., Ren, G.C., Sha, Z.X. and Shi, C.Y. 2004. Establishment of a continuous embryonic line from flounder Paralichthys olivaceus for virus isolation. Dis. Aquat. Org. 60: 241-246
Chen, S.L., Ren, G.C., Sha, Z.X. and Hong, Y. 2005. Development and characterization of a continuous embryonic cell line from turbot Scophthalmus maximus. Aquaculture 249: 63-68.
Chi, S.C., Hu, W.W. and Lo, B.J. 1999. Establishment and characterization of a continuous cell line (GF-1) derivered from grouper Ephinephelus coioides (Hamilton): a cell line susceptible to grouper nervous necrosis virus (GNNV). J. Fish Dis. 22:173-182.
Fryer, J.L. and Lannan, C.N. 1994. Three decades of fish cell culture: a current listing of cell lines derived from fishes. Journal of Tissue Culture Methods 10:57-94.
Goswami, M., Sharma, B.S., Tripathi, A.K., Yadav, K., Bahuguna, S.N., Nagpure, N.S., Lakra, W.S., Jena, J.K. 2012. Development and characterization of cell culture systems from Puntius (Tor) chelynoides (McClelland). Gene 500(1): 140-147.
Hameed, A.S. Sahul, Parameswaran, V., Shukla, R., Singh, I.S. Bright, Thirunavukkarasu, A.R. and Bhonde, R.R. 2006. Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass Lates calcarifer. Aquaculture 257 (1-4): 92-103.
Kumar, G.S., I.B.S. Singh and R. Philip 1998. Efficacy of fish and prawn muscle extracts as supplements of medium in development of primary cell culture system from larval tissue of aquarium fish, Poecilia reticulata. Indian J. Exp. Biol. 36: 91-94.
Kumar, G.S., I.B.S. Singh and R. Philip 2001. Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus). Aquaculture 194: 51-62.
Lakra, W.S., Bhonde, R.R. 1996. Development of primary cell culture from the caudal fin of an Indian major carp, Labeo rohita (Ham.). Asian Fish Sci. 9:149-152.
Lakra, W.S., Bhonde, R.R., Sivakumar, N., Ayyappan, S. 2006. A new fibroblast like cell line from the fry of golden mahseer Tor putitora (Ham). Aquaculture 253(1-4): 238-243.
Lakra, W.S., Sivakumar, N., Goswami, M., Bhonde, R.R. 2006. Development of two cell culture systems from Asian seabass Lates calcerifer (Bloch). Aquacult Res. 37:18-24.
Rao, K.S., Joseph, M.A., Shanker, K.M., Mohan, C.V. 1997. Primary cell culture from explants of heart tissue of Indian Major Carps. Curr. Sci. 73: 374-375.
Singh, N., Bahuguna S.N., Bhatt, K.C. 1993. The profile of river ecosystem, food and feeding habits of Hill-stream fishes and consequences of recent environmental degradation in garhwal Himalaya. Acta Ichthylologica,Vol.XXIII.
Sathe, P.S., Maurya, D.T., Basu, A., Gogate, S.S., Banerjee, K. 1995. Establishment and characterization of a new fish cell line, MG-3, from the gills of Mrigal Cirrhinus mrigala. Indian J. Exp. Biol. 33: 589-594.
Sathe, P.S., Basu, A., Mourya, D.T., Marathe, B.A., Gogate, S.S. and Banerjee, K. 1997. A cell line from the gill tissues of Indian cyprinoid, Labeo rohita. In Vitro Cell Dev. Biol. Anim. 33 (6): 425-427.