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Abstract

A primary cell culture has been established from the Snow trout Schizothorax plagiostomus. Primary cultures have developed from caudal fin tissues by explant culture technique. Cell outgrowth has been obtained from the caudal fin explant after 5-7 days of explant culture. The culture medium used for the experiment was Leibovitz-15 supplemented with 20% Fetal Bovine Serum. Radiation of cells from the explants started after 2 to 3 days. Both fibroblast like and epithelial like cells were noticed but fibroblast like cells dominated as the growth progressed.

Keywords

explant technique fetal bovine serum leibovitz-15media primary cell culture Schizothorax plagiostomus

Article Details

How to Cite
Chowdhary , A. K. ., & Bahuguna, S. N. . (2014). Development of primary cell culture from the caudal fin region of snow trout Schizothorax plagiostomus (Heckel). Environment Conservation Journal, 15(1&2), 149–152. https://doi.org/10.36953/ECJ.2014.151219

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