Main Article Content
Abstract
Terminalia chebula Retz. belongs to the family Combretaceae and is commonly known as Black myrobalan. In the present study fruits of Terminalia chebula were screened for potential antibacterial activity against Gram-negative bacterial strain Escherichia coli. The antibacterial activity was determined in aqueous, methanol, ethanol and acetone extracts by using disc diffusion ( Kirby-Bauer) method. The activity was carried out for different concentrations of plant extracts i.e. 100, 200 and 400 mg/ml and the relative solvents were used as control for respective extracts. Ciprofloxacin was used as positive control. The antibacterial activity of Terminalia chebula gave encouraging results.
Keywords
Article Details
Copyright (c) 2013 (C) ASEA
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
- Acharyya S, Patra A and Bag K P. 2009. Evaluation of the Antimicrobial Activity of some medicinal plants against Enteric Bacteria with Particular Reference to Multi-Drug Resistant Vibrio cholerae.Tropical Journal of Pharmaceutical Research, 8(3): 231-237.
- Akueshi CO, Kadiri CO, Akueshi EU, Agina SE and Ngurukwem B. 2002. Antimicrobial potentials of Hyptis sauvedens Poit (Lamiacae),Nigeria. J Bot ,15: 37-41.
- Bauer AW, Kirby WMN, Sherries IC and Turk M.1966. Antibiotic susceptibility testing by a standard single disc method. AMJ.Clin Path, 45: 493.
- Beyer H and Walter W.1997. Organic chemistry. 4th ed. , Published by Jerry March, pp.146-148.
- Chattopadhyay R R, Bhattacharya S K, Medd C, Chandas , Bag A. 2009. A Comparative evaluation of Antibacterial Potential of Some plants used in Indian traditional medicine for the treatment of microbial infections. Brazilian Archives of Biology and Technology, 52 (5) :1123-1128.
- Dhale DA and Markandeya SK. 2011. Antimicrobial and Phytochemical Screening of Plumbago zeylanica Linn. (Plumbaginaceae) Leaf. Journal of Experimental Sciences, 2 (3): 04-06.
- Erturk O, Kati H, Yayli N and Demurbau Z. 2006. Antimicrobial properties of Silene multifida(Adams) Rohrb., Plant extracts. Turk J Biol 30: 17-21.
- Farombi EO. 2003. African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents African Journal of Biotechnology, 2(12): 662-671.
- Hooker JD.1875-1897. Flora of British India. Vol. I-VII. L.Reeve & Co Ltd. Kent, England.
- Johnson JR.1991. Virulence factor in Escherichia coli urinay tract infections. Clin Microbiology Rev, 4(1): 80-128.
- Kaushik P and Chauhan A. 2009. Cynobacteria : Antibacterial Activity. New India Publishing Agency,Pitam Pura, New Delhi-110088, pp. xiii+1-198.
- Nataro J.P. and Kaper J.B.1998. Diarrheagenic Escherichia coli. Clin Microbiol Rev, 11 (1): 142-201.
- NCCLS- National Committee for Clinical Laboratory Standard.1997a. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. Approved Standard M7-A4, Wayne, Pa.
- Ncube NS, Afalayan AJ and Okoh A. 2008. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology 7(12): 17 97-1806.
- Saric C L, Cabarkapa S I, Beljkas M B, Misan C A, Sakac B M, Plavsic V D. 2009. Antimicrobial activity of Plant extracts from Serbia. Food and feed Research, 36 (1-2): 1-6.
- Segni L, Noureddine G, Amar Z, Labed B and Samir H. 2011. Phytochemical and Antibacterial Screening of some Algerian saharian medicinal plants. Plant sciences Feed 1(10): 179-182.
- Suriya J, Raja S, Bharathi VS and Rajasekaran R. 2012. Antimicrobial activity of some medicinal herbal extracts on clinically important bacterial pathogens. IJPSR,3(2): 490-493.
- Surono IS.2 004. Fermented Milk Probiotic and Healthcare. YAPMMI Jakarta, pp.1-252.
- Todar K. 2008. Bacterial structure in relation to pathogenicity: The importance of the bacterial surface. http://www.textbook of bacteriology.net University of Wisconsin-Madison Department of Bacteriology, pp.1-4
- Zar J H.1999. Biostatistical analysis. 4thed. Prentice Hall,Upper Saddle River, New Jersey, pp.662 .
References
Acharyya S, Patra A and Bag K P. 2009. Evaluation of the Antimicrobial Activity of some medicinal plants against Enteric Bacteria with Particular Reference to Multi-Drug Resistant Vibrio cholerae.Tropical Journal of Pharmaceutical Research, 8(3): 231-237.
Akueshi CO, Kadiri CO, Akueshi EU, Agina SE and Ngurukwem B. 2002. Antimicrobial potentials of Hyptis sauvedens Poit (Lamiacae),Nigeria. J Bot ,15: 37-41.
Bauer AW, Kirby WMN, Sherries IC and Turk M.1966. Antibiotic susceptibility testing by a standard single disc method. AMJ.Clin Path, 45: 493.
Beyer H and Walter W.1997. Organic chemistry. 4th ed. , Published by Jerry March, pp.146-148.
Chattopadhyay R R, Bhattacharya S K, Medd C, Chandas , Bag A. 2009. A Comparative evaluation of Antibacterial Potential of Some plants used in Indian traditional medicine for the treatment of microbial infections. Brazilian Archives of Biology and Technology, 52 (5) :1123-1128.
Dhale DA and Markandeya SK. 2011. Antimicrobial and Phytochemical Screening of Plumbago zeylanica Linn. (Plumbaginaceae) Leaf. Journal of Experimental Sciences, 2 (3): 04-06.
Erturk O, Kati H, Yayli N and Demurbau Z. 2006. Antimicrobial properties of Silene multifida(Adams) Rohrb., Plant extracts. Turk J Biol 30: 17-21.
Farombi EO. 2003. African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents African Journal of Biotechnology, 2(12): 662-671.
Hooker JD.1875-1897. Flora of British India. Vol. I-VII. L.Reeve & Co Ltd. Kent, England.
Johnson JR.1991. Virulence factor in Escherichia coli urinay tract infections. Clin Microbiology Rev, 4(1): 80-128.
Kaushik P and Chauhan A. 2009. Cynobacteria : Antibacterial Activity. New India Publishing Agency,Pitam Pura, New Delhi-110088, pp. xiii+1-198.
Nataro J.P. and Kaper J.B.1998. Diarrheagenic Escherichia coli. Clin Microbiol Rev, 11 (1): 142-201.
NCCLS- National Committee for Clinical Laboratory Standard.1997a. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. Approved Standard M7-A4, Wayne, Pa.
Ncube NS, Afalayan AJ and Okoh A. 2008. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology 7(12): 17 97-1806.
Saric C L, Cabarkapa S I, Beljkas M B, Misan C A, Sakac B M, Plavsic V D. 2009. Antimicrobial activity of Plant extracts from Serbia. Food and feed Research, 36 (1-2): 1-6.
Segni L, Noureddine G, Amar Z, Labed B and Samir H. 2011. Phytochemical and Antibacterial Screening of some Algerian saharian medicinal plants. Plant sciences Feed 1(10): 179-182.
Suriya J, Raja S, Bharathi VS and Rajasekaran R. 2012. Antimicrobial activity of some medicinal herbal extracts on clinically important bacterial pathogens. IJPSR,3(2): 490-493.
Surono IS.2 004. Fermented Milk Probiotic and Healthcare. YAPMMI Jakarta, pp.1-252.
Todar K. 2008. Bacterial structure in relation to pathogenicity: The importance of the bacterial surface. http://www.textbook of bacteriology.net University of Wisconsin-Madison Department of Bacteriology, pp.1-4
Zar J H.1999. Biostatistical analysis. 4thed. Prentice Hall,Upper Saddle River, New Jersey, pp.662 .