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July 28, 2023
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October 20, 2023
Published
January 10, 2024
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Abstract

Nanoparticles are synthesized by using various chemical methods in higher yields but they are not very environmentally friendly and have hazardous effects on living cells. This can be attributed to the overuse of hazardous chemicals involved in the process. The green approach of nanoparticle synthesis is widely gaining attention worldwide as it is considered as nontoxic, harmless and ecofriendly. Apart from this they also have multiple applications in various fields of science and technology. Nanoparticles synthesized by using various phytochemicals are also effective against a variety of microbial populations. The objective of this study is to synthesize silver nanoparticles (AgNPs) from the fruit extracts of Wrightia tinctoria and evaluating its antimicrobial capacities against gram-positive and negative bacterial strains. Silver nanoparticles were synthesized using different solvent extracts of Wrightia tinctoria pods. The formation of silver nanoparticles was noted by detecting the change in color of the solution. The presence of nanoparticles was detected by performing UV visible spectroscopy and monitoring the spectrum from 400 nm to 800 nm. A small peak at 425 nm suggested the presence of silver nanoparticles. In a later part of the study, the inhibitory effect of green synthesized silver nanoparticles on the growth of E. coli and S. aureus was monitored. The appearance of a zone of inhibition further confirmed the antimicrobial effect of the synthesized nanoparticles.

Keywords

Antimicrobial activity Green synthesis Nanoparticles Silver nanoparticles Silver nitrate Wrightia tinctoria

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Durge , A. A., Dongre, U., & Moon, U. R. (2024). Antimicrobial activity of silver nanoparticles synthesized from Wrightia tinctoria fruit extracts. Environment Conservation Journal, 25(1), 16–21. https://doi.org/10.36953/ECJ.26582638
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References

  1. Adeyemi, J. O., Elemike, E. E., & Onwudiwe, D. C. (2019). ZnO nanoparticles mediated by aqueous extracts of Dovyalis caffra fruits and the photocatalytic evaluations. Materials Research Express, 6(12), 125091. DOI: https://doi.org/10.1088/2053-1591/ab5bcb
  2. Agnihotri, S., Mukherji, S., & Mukherji, S. (2014). Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy. Rsc Advances, 4(8), 3974-3983. DOI: https://doi.org/10.1039/C3RA44507K
  3. Ankamwar, B. (2010). Biosynthesis of gold nanoparticles (green-gold) using leaf extract of Terminalia catappa. Journal of Chemistry, 7, 1334-1339. DOI: https://doi.org/10.1155/2010/745120
  4. Banerjee, N., Biswas, S., Hossain, C. M., & Basak, P. (2022). Effectiveness of onion (Allium cepa L.) skin in human health. In Contemporary Medical Biotechnology Research for Human Health (pp. 115-125). Academic Press. DOI: https://doi.org/10.1016/B978-0-323-91251-8.00003-9
  5. Bapat, R. A., Chaubal, T. V., Joshi, C. P., Bapat, P. R., Choudhury, H., Pandey, M., ... & Kesharwani, P. (2018). An overview of application of silver nanoparticles for biomaterials in dentistry. Materials Science and Engineering: C, 91, 881-898. DOI: https://doi.org/10.1016/j.msec.2018.05.069
  6. Cavassin, E. D., de Figueiredo, L. F. P., Otoch, J. P., Seckler, M. M., de Oliveira, R. A., Franco, F. F., & Costa, S. F. (2015). Comparison of methods to detect the in vitro activity of silver nanoparticles (AgNP) against multidrug resistant bacteria. Journal of nanobiotechnology, 13(1), 1-16. DOI: https://doi.org/10.1186/s12951-015-0120-6
  7. Elamawi, R. M., Al-Harbi, R. E., & Hendi, A. A. (2018). Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi. Egyptian journal of biological pest control, 28(1), 1-11. DOI: https://doi.org/10.1186/s41938-018-0028-1
  8. Gong, P., Li, H., He, X., Wang, K., Hu, J., Tan, W., ... & Yang, X. (2007). Preparation and antibacterial activity of Fe3O4@ Ag nanoparticles. Nanotechnology, 18(28), 285604. DOI: https://doi.org/10.1088/0957-4484/18/28/285604
  9. Husen, A. (2017). Gold nanoparticles from plant system: synthesis, characterization and their application. Nanoscience and plant–soil systems, 455-479. DOI: https://doi.org/10.1007/978-3-319-46835-8_17
  10. Javed, R., Zia, M., Naz, S., Aisida, S. O., Ain, N. U., & Ao, Q. (2020). Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects. Journal of Nanobiotechnology, 18, 1-15. DOI: https://doi.org/10.1186/s12951-020-00704-4
  11. Khandel, P., Yadaw, R. K., Soni, D. K., Kanwar, L., & Shahi, S. K. (2018). Biogenesis of metal nanoparticles and their pharmacological applications: present status and application prospects. Journal of Nanostructure in Chemistry, 8, 217-254. DOI: https://doi.org/10.1007/s40097-018-0267-4
  12. Khorrami, S., Zarrabi, A., Khaleghi, M., Danaei, M., & Mozafari, M. R. (2018). Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. International journal of nanomedicine, 8013-8024. DOI: https://doi.org/10.2147/IJN.S189295
  13. Kishore, K., Prasad, P., Selvasudha, N., Rajesh, S., & Vasanthi, H. R. (2023). Silver Nanoparticle Synthesized with Fenugreek Leaves Is Biologically Potent than Chemically Synthesized Nanoparticle. Journal of Pharmaceutical Innovation, 1-14. DOI: https://doi.org/10.1007/s12247-023-09769-8
  14. Kumari, B. (2018). A Review on Nanoparticles: Their Preparation method and applications. Ind Res J Pharm Sci, 5(2), 1420. DOI: https://doi.org/10.21276/irjps.2018.5.2.3
  15. Lu, Z., Rong, K., Li, J., Yang, H., & Chen, R. (2013). Size-dependent antibacterial activities of silver nanoparticles against oral anaerobic pathogenic bacteria. Journal of Materials Science: Materials in Medicine, 24, 1465-1471. DOI: https://doi.org/10.1007/s10856-013-4894-5
  16. Meroni, G., Soares Filipe, J. F., & Martino, P. A. (2020). In vitro antibacterial activity of biological-derived silver nanoparticles: Preliminary data. Veterinary sciences, 7(1), 12. http://dx.doi.org/10.3390/vetsci7010012 DOI: https://doi.org/10.3390/vetsci7010012
  17. Mittal, A. K., Chisti, Y., & Banerjee, U. C. (2013). Synthesis of metallic nanoparticles using plant extracts. Biotechnology advances, 31(2), 346-356. https://doi.org/10.1016/j.biotechadv.2013.01.003 DOI: https://doi.org/10.1016/j.biotechadv.2013.01.003
  18. Mohanraj, V. J., & Chen, Y. J. T. J. O. P. R. (2006). Nanoparticles-a review. Tropical journal of pharmaceutical research, 5(1), 561-573. DOI: https://doi.org/10.4314/tjpr.v5i1.14634
  19. Morones-Ramirez, J. R., Winkler, J. A., Spina, C. S., & Collins, J. J. (2013). Silver enhances antibiotic activity against gram-negative bacteria. Science translational medicine, 5(190), 190ra81-190ra81. DOI: https://doi.org/10.1126/scitranslmed.3006276
  20. Murei, A., Ayinde, W. B., Gitari, M. W., & Samie, A. (2020). Functionalization and antimicrobial evaluation of ampicillin, penicillin and vancomycin with Pyrenacantha grandiflora Baill and silver nanoparticles. Scientific Reports, 10(1), 11596. DOI: https://doi.org/10.1038/s41598-020-68290-x
  21. Pandey, M. M., Rastogi, S., & Rawat, A. K. S. (2013). Indian traditional ayurvedic system of medicine and nutritional supplementation. Evidence-Based Complementary and Alternative Medicine, 2013. DOI: https://doi.org/10.1155/2013/376327
  22. Pomal, N. C., Bhatt, K. D., Modi, K. M., Desai, A. L., Patel, N. P., Kongor, A., & Kolivoška, V. (2021). Functionalized silver nanoparticles as colorimetric and fluorimetric sensor for environmentally toxic mercury ions: an overview. Journal of fluorescence, 31, 635-649. DOI: https://doi.org/10.1007/s10895-021-02699-z
  23. Sowmya, C., Lavakumar, V., Venkateshan, N., Ravichandiran, V., & Saigopal, D. V. R. (2018). Exploration of Phyllanthus acidus mediated silver nanoparticles and its activity against infectious bacterial pathogen. Chemistry Central Journal, 12(1), 1-9. DOI: https://doi.org/10.1186/s13065-018-0412-7
  24. Rai, M., Yadav, A., & Gade, A. (2009). Silver nanoparticles as a new generation of antimicrobials. Biotechnology advances, 27(1), 76-83. DOI: https://doi.org/10.1016/j.biotechadv.2008.09.002
  25. Zaheer, Z., Malik, M. A., Al-Nowaiser, F. M., & Khan, Z. (2010). Preparation of silver nanoparticles using tryptophan and its formation mechanism. Colloids and Surfaces B: Biointerfaces, 81(2), 587-592. DOI: https://doi.org/10.1016/j.colsurfb.2010.08.001
  26. Zhang, X. F., Liu, Z. G., Shen, W., & Gurunathan, S. (2016). Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. International journal of molecular sciences, 17(9), 1534. DOI: https://doi.org/10.3390/ijms17091534