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In the present study twenty-eight hybrid combinations resulting from the half-diallel mating of eight quality protein maize (QPM) inbred lines were chosen in order to examine the potential to combine and gene activity for ten yield and component attributes. As part of the All India Coordinated Research Project (AICRP) on maize during the kharif-2020 season, the experiment was done at the research farm of college of Agriculture, Odisha University of Agriculture & Technology (OUAT) Bhubaneswar, using a randomized complete block design replicated thrice. Every observation recorded was subjected to statistical evaluation and it was revealed that the mean squares derived from the general combining ability (gca) and specific combining ability (sca) were highly significant (p ≥0.01). Estimates of sca effects   were greater than that of gca effects for all of the variables used in the study, suggesting to the predominance of dominant gene action. The inbred lines Q4-DQL 2221-1-1(833.792), Q2-DQL 2099 (517.658) and Q3- DQL 2159 (350.325) shows high gca effects for yield and its attributing traits due to more additive gene action thus identified as good general combiners for yield. Twelve of the twenty-eight crosses showed significant (p ≥0.01 and 0.05) positive sca effect on grain yield. The best experimental crosses for grain yield based on per se performance and sca effects were Q2 x Q8 (2106.748), Q1 x Q6 (2053.048), Q3 x Q7 (2027.082), and Q3 x Q6 (1719.884).


Diallel mating General combining ability Gene action Quality protein maize Specific combining ability

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Agarwal, A. K., Swain, D., Lenka, D., Kumar, A., & Tripathy , S. K. (2024). Gene actions and combining ability effects on grain yield and its constituent traits in inbred lines of quality protein maize . Environment Conservation Journal, 25(1), 250–256.


  1. Agrawal, P. K., Mallikarjuna, M. G., & Gupta, H. S. (2018). Genetics and applied genomics of quality protein maize for food and nutritional security. Biotechnologies of Crop Improvement, Volume 3: Genomic Approaches, 151-178. DOI:
  2. Ali, M. (2020). Diallel analysis of maize inbreds for grain yield, protein and tryptophan content. Egyptian Journal of Agronomy, 42(1), 1-17. DOI:
  3. Arunkumar, B., Gangapp, E., & Ramesh, S. (2022). Combining ability estimates and gene action studies from full diallel mating design in maize (Zea mays L.). Annals of Plant and Soil Research, 24(1), 127-136. DOI:
  4. Bahari, M., Rafii, M. Y., Saleh, G. B., & Latif, M. A. (2012). Combining ability analysis in complete diallel cross of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). The Scientific World Journal, 2012. DOI:
  5. Basser, P., Marker, S., & Patil, K. S. (2021). Combining ability analysis of early maturing Quality Protein Maize (Zea mays L) lines and heterosis of their F1 hybrids. International Journal of Current Microbiology and Applied Sciences, 10(02), 2065-2075. DOI:
  6. Das, A. K., Gowda, M. M., Muthusamy, V., Zunjare, R. U., Chauhan, H. S., Baveja, A., ... & Hossain, F. (2021). Development of maize hybrids with enhanced vitamin-E, vitamin-A, lysine, and tryptophan through molecular breeding. Frontiers in plant science, 12, 659381. DOI:
  7. El-Shamarka, S. A., Abdel-Sattar, M., & El-Nahas, M. (2015). Heterosis and combining ability for yield and its components through diallel cross analysis in maize (Zea mays L.). Alex. J. Agric. Res, 60(2), 87-94.
  8. Griffing, B. R. U. C. E. (1956). Concept of general and specific combining ability in relation to diallel crossing systems. Australian journal of biological sciences, 9(4), 463-493. DOI:
  9. Hassan, A. A., Jama, A. A., Mohamed, O. H., & Biswas, B. K. (2019). Study on combining ability and heterosis in maize (Zea mays L.) using partial diallel analysis. Inter. J. of Plant Breed. And Crop Sci, 6(2), 520-526.
  10. Hemalatha, V., Kumar, R. S., Swarnalatha, V., & Suresh, J. (2014). Combining ability and gene action for morphological parameters in quality protein maize (Zea mays L.). International Journal of Plant, Animal and Environmental Sciences, 4(2), 230-235.
  11. Kamal, N., Khanum, S., Siddique, M., Saeed, M., Ahmed, M. F., Kalyar, M. T. A., ... & Mahmood, B. (2023). Heterosis and Combining Ability Studies in A 5x5 Diallel Crosses of Maize Inbred Lines. Journal of Applied Research in Plant Sciences, 4(01), 419-424. DOI:
  12. Karim, A. N. M. S., Ahmed, S., Akhi, A. H., Talukder, M. Z. A., & Karim, A. (2018). Combining ability and heterosis study in maize inbreds throughout diallel mating design. Bangladesh Journal of Agricultural Research, 43(4), 599-609. DOI:
  13. Krupakar, A., Kumar, B., & Marker, S. (2013). Combining ability for yield and quality traits in single cross hybrids of maize (Zea mays L.). The Bioscan, 8(4), 1347-1355.
  14. Kumar, A., Dadheech, A., Kiran, N., Bisen, P., & Kumar, S. (2017). Diallel Analysis of Combining Ability for Yield and Yield Contributing Traits over the Environments in Maize (Zea mays L.). Int. J. Curr. Microbiol. App. Sci, 6(10), 196-208. DOI:
  15. Netravati, G., Limbikai, T., Hiremath, M. N., & Adiger, S. (2014). Combining ability studies for grain yield and quality parameters in newly developed maize (Zea mays L.) inbred line crosses. Electronic Journal of Plant Breeding, 5(4), 771-774.
  16. Patel, P. C., & Kathiria, K. B. (2016). Heterosis and Combining ability for yield and quality traits in Quality Protein Maize (Zea mays L.). Electronic Journal of Plant Breeding, 7(4), 960-966. DOI:
  17. Poehlman, J. M. (2006). Breeding Field Crops. 5th Edn. The AVI publish. Co. Inc. Westport, Connecticut.
  18. Sandesh, G. M., Karthikeyan, A., Kavithamani, D., Thangaraj, K., Ganesan, K. N., Ravikesavan, R., & Senthil, N. (2018). Heterosis and combining ability studies for yield and its component traits in Maize (Zea mays L.). Electronic Journal of Plant Breeding, 9(3), 1012-1023. DOI:
  19. Scaria, S., Marker, S., Raj, G. S., & Karthika, A. (2020). Genetic evaluation of quality protein maize hybrids for heterosis and combining ability for grain yield and its component characters. Journal of Pharmacognosy and Phytochemistry, 9(2), 1101-1105.
  20. Shinde, N. A., Bharose, A. A., Sarode, D. K., Swathi, R. S., Pimpale, P. A., & Shinde, S. S. (2021). Assessment of hybrid purity in maize (Zea mays L.) using RAPD and SSR markers. Pharma Innov J, 10, 870-874.
  21. Singh, P. K., Ranjan, R., Shahi, J. P., & Singh, A. K. (2014). Studies on combining ability analysis in white kernel colour of quality protein maize (Zea mays L.). Agricultural Science Digest-A Research Journal, 34(1), 21-25. DOI:
  22. Sprague, G. F., & Tatum, L. A. (1942). General vs. specific combining ability in single crosses of corn 1. Agronomy journal, 34(10), 923-932. DOI:
  23. Tilahun, B., Dida, M., Deressa, T., Garoma, B., Demissie, G., Kebede, D., ... & Chere, A. T. (2017). Combining ability analysis of quality protein maize (QPM) inbred lines for grain yield, agronomic traits and reaction to grey leaf spot in mid-altitude areas of Ethiopia.
  24. Yerva, S. R., Sekhar, T. C., Allam, C. R., & Krishnan, V. (2016). Combining ability studies in maize (Zea mays L.) for yield and its attributing traits using Griffing’s diallel approach. Electronic Journal of Plant Breeding, 7(4), 1046-1055. DOI: