Main Article Content


The present investigation aimed to determine the per cent growth inhibition of different fungicides against Alternaria burnsii, the causal agent of cumin blight. The study was conducted during 2020-21 at the Experiential Unit of Plant Pathology, College of Agriculture, SKRAU, Bikaner. Using the poisoned food technique, eleven fungicides were tested at varying concentrations (100, 200, 300, and 500 ppm). After seven days of incubation, the radial growth and per cent growth inhibition of A. burnsii were measured. The results revealed that increasing the fungicide concentration led to greater inhibition of mycelium growth. Among the tested fungicides, Tebuconazole 50% + Trifloxystrobin 20% WG exhibited the highest mean inhibition (76.94%), followed by Tebuconazole 2DS (65.09%) and Pyraclostrobin 13.30% + Epoxiconazole 5% SE (58.75%). Notably, Tebuconazole 50% + Trifloxystrobin 20% WG at concentrations of 300 ppm and 500 ppm, as well as Tebuconazole 2DS at 500 ppm, demonstrated the highest effectiveness with cent per cent growth inhibition. On the other hand, Chlorothalonil showed the least mean growth inhibition (22.96%). The results demonstrated that as the fungicide concentration increased, there was a corresponding increase in the inhibition of A. burnsii growth. These findings highlight the potential effectiveness of selected fungicides, particularly Tebuconazole 50% + Trifloxystrobin 20% WG and Tebuconazole 2DS for managing cumin blight caused by A. burnsii.


Growth inhibition Incubation Per cent growth inhibition Poisoned food technique Radial growth

Article Details

How to Cite
Varma, S., & Kumhar, D. R. (2023). In-vitro evaluation of fungicides against Alternaria burnsii (Uppal, Patel and Kamat) causing blight of cumin (Cuminum cyminum L.) . Environment Conservation Journal, 25(1), 50–55.


  1. Bliss, C. I. (1934). The method of probits. Science, 79(2037), 38–39. doi: 10.1126/science.79.2037.38 DOI:
  2. Dange, S. (1995). Diseases of cumin (Cuminum cyminum L.) and their management. Journal of Spices and Aromatic Crops, 4(1), 57–60.
  3. Gemawat, P. D., & Prasad, N. (1972). Epidemiological studies on Alternaria blight of cuminum cyminum. Indian Journal of Mycology and Plant Pathology, 2(1), 65–75.
  4. Jagani, B., Parmar, M., Patel, V. N., & Vishwakarma, N. P. (2023). Antifungal activity of some plant extracts on Alternaria burnsii: the causal agent of Alternaria blight of cumin. Sustainability, Agri, Food and Environmental Research, 11, 1–6.
  5. Jia, k., Chen, G., Zeng, G., Liu, F., Liao, X., Guo, C., Luo, J., Xiong, G., & Lu, H. (2020). Low trifloxystrobin-tebuconazole concentrations induce cardiac and developmental toxicity in zebrafish by regulating notch mediated-oxidative stress generation. Ecotoxicology and Environmental Safety, 24, 1–9.
  6. Kakraliya, G. L., Ahir, R. R., Yadav, A. L., Choudhary, S., Jat, M. K. & Yadav, S. L. (2021). Effect of fungicides on Alternaria blight of cumin caused by Alternaria burnsii. International Journal of Agriculture Sciences, 13(9), 10818–10820.
  7. Nene, Y. L., & Thapliyal, P. N. (1993). Fungicides in plant disease control, Oxford and IBH Publication Company. New Delhi. 331 p.
  8. Patel, R. J. (1968). Alternaria blight and powdery mildew of cumin (Cuminum cyminnum L.) and their management. M.Sc. Thesis, Gujarat Agriculture University, Gujarat, India.
  9. Pipliwal, S. K., Jadeja, K., & Sharma, A. (2017). Effect of different fungicides and phytoextracts on growth inhibition of Alternaria burnsii. Annals of Agricultural Research, 38(2), 230–234.
  10. Polra, M. H., & Jadeja, K. B. (2011). Evaluation and integration of fungicides, phytoextracts and bioagents against Alternaria burnsii causing cumin blight. The Journal of Mycology and Plant Pathology, 41(1), 126–131.
  11. Pranaya, K., Bhat, B. N., Devi, G. M., & Triveni, S. (2020). In vitro evaluation of fungicides against Alternaria leaf spot of cotton. International Journal of Chemical Studies, 8(4), 3571–3575. DOI:
  12. Rajvanshi, N. K., Singh, H. K., & Maurya, M. K. (2020). Efficacy of newer molecules of fungicides against Alternaria brassicae in vitro. International Journal of Current Microbiology and Applied Sciences, 9(9), 3150–3154. DOI:
  13. Saha, S., Hingmire, S., Shabeer, T. P. A., Banerjee, K., Ashtekar, N., Patil, A., & Rai, A. B. (2018). Assessment of Trifloxystrobin 25% + Tebuconazole 50%-75 WG bio efficacy, safety and residue dynamics against leaf spot of cabbage. Chemical Science Review and Letters, 7(28), 867–874.
  14. Shakir, A. S., Mirza, J. H., Sahi, S. T., & Ansar, M. (1995). First report of Alternaria burnsii, the causal organism of cumin blight in Pakistan. Pakistan Journal of Phytopathology, 7(2), 219 p.
  15. Shekhawat, N., Trivedi, A., Kumar, A., & Sharma, S. K. (2013). Management of Alternaria burnsii causing blight of cumin. International Journal of Plant Protection, 6(2), 280–284.
  16. Uppal, B. N., Patel, M. K., & Kamat, M. N. (1938). Alternaria blight of cumin. Indian Journal of Agricultural Science, 8(1), 49–62.
  17. Wadud, A., Das, S. S., & Khokon, M. A. R. (2021). Prevalence of the Alternaria blight of cumin (Cuminum cyminum L.) in Bangladesh: morphology, phylogeny and pathogenic variation of Alternaria spp. Saudi Journal of Biological Sciences, 28(10), 5865–5874. DOI:
  18. Yadav, P. C., Gahlot N., Bijarnia, A. L., Kumar, A., & Choudhary, M. (2022). Efficacy of Tebuconazole 25.9 EC against cumin blight (Alternaria burnsii). Chemical Science Review and Letters, 11(43), 385–387.