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High temperature affects various physiological processes of the plant. Delayed sowing and changing climate both subject the crop to increasing temperatures during the crop growth period. There is a need to take on a technique to screen the wide number of genotypes for high-temperature tolerance. In the present study, a screening protocol was followed based on the principle of “acquired tolerance” in which 47 sunflower seedlings were exposed to sub-lethal heat stress to induce tolerance before subjecting to subsequent lethal stress and the second set were directly exposed to lethal stress. Significant variation was observed for the traits - survival percentage, total seedling length, and seedling weight. Tolerant inbreds were identified using Z distribution and PCA. Results suggested that TIR is a rapid and powerful technique that can be used to screen large number of germplasms to identify thermotolerant lines.


Lethal Sunflower Sublethal Survival Temperature Thermotolerance

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How to Cite
V, A., Prayaga, L., C, S., & Guhe, A. (2024). Evaluating thermotolerant sunflower genotypes with temperature induction response (TIR) technique . Environment Conservation Journal, 25(1), 175–183.


  1. Debaeke, P., Bedoussac, L., Bonnet, C., Bret-Mestries, E., Seassau, C., Gavaland, A., Raffaillac, D., Tribouillois, H., Véricel, G & Justes, E. (2017). Sunflower crop: Environmental-friendly and agroecological. OCL Oilseeds Fats Crops Lipids, 24 (3): 304 DOI:
  2. Harihar, S., Srividhya, S., Vijayalakshmi, C., & Boominathan, P. (2014). Temperature induction response technique—a physiological approach to identify thermotolerant genotypes in rice. International Journal of Agricultural Sciences, 10, 230-232.
  3. Hasanuzzaman, M., Hossain, M.A., da Silva, J.A.T & Fujita, M. (2012). Plant Responses and Tolerance to Abiotic Oxidative Stress: Antioxidant Defenses is a Key Factor. In Crop Stress and Its Management: Perspectives and Strategies; Springer: Berlin, Germany, pp. 261–316. DOI:
  4. Kheir, E.A., Sheshshayee, M.S., Prasad, T. G. & Udayakumar, M. (2012). Temperature Induction Response as a Screening Technique for Selecting High Temperature-Tolerant Cotton Lines. The Journal of Cotton Science, 16:190–199.
  5. Moreno, A.A & Orellana, A. (2011). The physiological role of the unfolded protein response in plants. Biological Research. 44, 75–80. DOI:
  6. Partheeban, C., Vijayaraghavan, H., Sowmyapriya, S., Srividhya, S & Vijayalakshmi, D. (2017). Temperature induction response and accumulation of starch granules as indices to identify the thermotolerance of pulses at early growth stages. Legume Research. 40: 655-65 DOI:
  7. Qadir, G., Hassan, F.U & Malik, M.A. (2007). Growing degree days and yield relationship in sunflower (Helianthus annus L.). International Journal of Agriculture and Biology, 9: 564-568.
  8. Senthil-Kumar, M., Kumar, G., Srikanthbabu, V & Udayakumar, M. (2007). Assessment of variability in acquired thermotolerance: potential option to study genotypic response and the relevance of stress genes. Journal of Plant Physiology, 164:111-125. DOI:
  9. Sunflower ACRIP annual report, (2014-2015). p. 8
  10. Sunflower Statistics (2019). FAOSTAT (Source:
  11. Vidya, S.M., Laxman, R.H., Bhatt, R.M.; Rekha, A., John Sunoj and Ravishankar K.V. 2017. Temperature induction response technique: a screening tool for evaluation of banana cultivars for thermotolerance. Indian journal of plant physiology, 22 (1): 79-84 DOI:
  12. Vijayalakshmi, D., Srividhya, S., Vivitha, P., & Raveendran, M. (2015). Temperature induction response (TIR) as a rapid screening protocol to dissect the genetic variability in acquired thermotolerance in rice and to identify novel donors for high temperature stress tolerance. Indian Journal of Plant Physiology, 20: 368-374. DOI: