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Abstract
Methanol, diethyl ether and acetone extracts of leaf of itex negundo were tested for their antibacterial activity against two human pathogenic bacteria Staphylococcus aureus, Pseudomonas aeruginosa and one fungus Aspergillus niger using the disc diffusion method. It was found that all the extracts produced inhibitory effect but the methanol extract of leaves exhibited a superior level of antimicrobial activity. The minimum inhibitory concentration(M IC) and minimum fungicidal concentration(MFC)was determined by broth dilution method. The results were compared with the reference antibiotics.
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References
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References
Alonso, Paz E, Cerdeiras, MP., Fernandez, J., Ferreira, F., Moyna, P., Soubes, M., Vazquez, A., Vero, S.,and Zunino, L., 1995. Screening of Uruguayan medicinal plants for antimicrobial activity. J. Ethnopharmacology, 45: 67-70. DOI: https://doi.org/10.1016/0378-8741(94)01192-3
Anon, 1976. Wealth ofIndia. Raw materials, CSIR. New Delhi. Vol. x pp: 522.
Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Truck, 1966. Antibiotic susceptibility testing by standardized single disc method. American Journal of Clinical Pathology, 45: 493. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
Covington, R.T., 1988. Management of Diarrhoea. Fact and Comparision. Drug News Letter 13:1-2.
Bailley and Scott's Diagnostic Microbiology,
Mosby Publication USA, 1O Edition. pp: 252-258. Kandil, O., Radwan, N.M., Hassan, A.B., Ames, A.M.M., Bana, H.A.., and Ames W.M.M., 1994.
Extracts and fractions of Thymus capitatus exhibit antimicrobial activities. J. Ethnopharmacology, 44:19-24. DOI: https://doi.org/10.1016/0378-8741(94)90094-9
Sashikumar, J.M., Remya, M., Janardhanan, K.(2003) Nilgiri Biosphere reserve and Western ghats. Asian Journal ofMicrobial Tech. En. Sci., 5:183-185.