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Abstract
The bark of Ficus religiosa L., Ficus benghalensis L., Ficus gloomerata L., fruits of Tribulus terrestris L. and roots of Saussurea lappa Clarke. were investigated for in vitro antibacterial activity. The various solvent extracts like petroleum ether, chloroform, aqueous and methanol of plants were screened against Neisseria gonorrhoeae isolated from the patients suffering from vaginitis. The extracts were subjected for antibacterial activity against the pathogen at 200mg/ml concentration by agar well diffusion and agar disc diffusion method. The results of antibacterial activity revealed that methanol extracts of all the plants exhibits good activity as compared to petroleum ether, chloroform and aqueous extracts. The antibacterial activities of extracts were compared with standard antibiotic cefotaxime. The MIC of the methanol extract of all the plants was also calculated against the pathogen.
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References
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- National Committee for Clinical Lab. Standards (NCCLS ) 1992. Proposed standard document. M- 27.
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- Ody, P., 2000. The Complete Guide of Medicinal Herbal. London : Dorling Kindersley. pp: 223.
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- Perez, C., Pauli, M. and Bazerque, P., 1990. An antibiotic assay by the well agar diffusion method. Acta Biol. Et. Med. Exper., 15: 113-115 .
- Ray, P.G. and Majumdar, S.K., 1976. Antimicrobial activity of some Indian medicinal plants. Journal of Economic Botany, 30(4): 317-320. DOI: https://doi.org/10.1007/BF02904653
- Warrier, D.K., Nambiar, V.P.K. and Ramankutty, C., 1995. Indian Medicinal Plants. Orient Longman Ltd, Madras., pp: 1-5.
References
Ahmad, I., Mehmood, Z. and Mohmmad, F., 1998 .Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology, 62: 183-193. DOI: https://doi.org/10.1016/S0378-8741(98)00055-5
Bauer, A.W. and Kirby, W.M., 1966. Antibiotic susceptibility testing by a standardized single disk method. Ame. J. Clin. Pathol., 45: 493-496. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
Bhattacharjee, S.K., 1998. Handbook of medicinal plants.
Pointer Pub, Jaipur-03, India. pp: 1-6.
Burneti, R.W., Haber, M.H., Hackel, E., Hanson, C.A., Keron, D.F. and Lee Lewandrowski, E., 1994. Clinical laboratory medicine, eds.Williams & Wilkins, Philadelphia U.S. pp: 1113-1120.
Chah, K.F., Eze, C.A., Emuelosi, C.E and Esimone, C.O., 2006. Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants. Journal of Ethnopharmacology, 104: 164-167. DOI: https://doi.org/10.1016/j.jep.2005.08.070
Iwu, M.W., Duncan, A.R. and Okunji, C.O., 1999. New antimicrobials of plant origin. In: Janick J. Perspectives on New Crops and New Uses. ASHS Press, Alexander, VA. 457-462.
National Committee for Clinical Lab. Standards (NCCLS ) 1992. Proposed standard document. M- 27.
Nair, R. and Chanda, S., 2007. Antimicrobial activities of some medicinal plants of the Western region of India. Turkish J. Biol., 31: 231-236.
Ody, P., 2000. The Complete Guide of Medicinal Herbal. London : Dorling Kindersley. pp: 223.
Parekh, J. and Chanda, S., 2007 . Antimicrobial and Phtyochemical studies on twelve spp. of Indian medicinal plants. African Journal of Biomedical research, 10: 175- 181. DOI: https://doi.org/10.4314/ajbr.v10i2.50624
Perez, C., Pauli, M. and Bazerque, P., 1990. An antibiotic assay by the well agar diffusion method. Acta Biol. Et. Med. Exper., 15: 113-115 .
Ray, P.G. and Majumdar, S.K., 1976. Antimicrobial activity of some Indian medicinal plants. Journal of Economic Botany, 30(4): 317-320. DOI: https://doi.org/10.1007/BF02904653
Warrier, D.K., Nambiar, V.P.K. and Ramankutty, C., 1995. Indian Medicinal Plants. Orient Longman Ltd, Madras., pp: 1-5.