Main Article Content


Sesame is the oldest oilseed crop in agriculture, and it produces more oil than any other crop on the planet. This research aimed to investigate the impact of different oil extraction procedures on sesame seed oil physicochemical quality and oil recovery. The oil was extracted from the clean and healthy seeds using four extraction methods: aqueous, enzyme-assisted aqueous, ultrasound-assisted aqueous and solvent extraction using the Soxhlet apparatus. It was observed that ultrasound-assisted aqueous extracted oil had maximum saponification value and minimum acid value, refractive index, and lower free fatty acid value, compared with aqueous extracted oil and enzyme-assisted aqueous extracted oil. Ultrasound-assisted aqueous extraction method also yielded maximum oil, retrieval followed by enzyme-assisted aqueous extraction and aqueous extraction.


enzyme-assisted aqueous solvent extraction Sesame oil Ultrasound-assisted aqueous

Article Details

How to Cite
Singh, G., Kumar, M., Zalpouri, R., Potdar, P. P., Singh, K., & Kaur, K. (2023). Effects of different aqueous extraction techniques on physicochemical quality and oil recovery of sesame oil. Environment Conservation Journal, 24(1), 136–142.


  1. Abdulkarim, S. M., Long, K., Lai, O. M., Muhammad, S. K. S., & Ghazali, H. M. (2005). Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chemistry, 93(2), 253–263. DOI:
  2. Anonymous. (2021a). Production volume of sesame across India from financial year 2013 to 2019, with estimates until 2021. Online available at (Assessed on 12 September 2021)
  3. Anonymous. (2021b). Agricultural Statistics of Land Classification in Punjab State. Online available at on 12 September 2021)
  4. AOAC (Association of Official Analytical Chemists). (1990). Official Method of Analysis. 15th Edn, Association of Official Analytical Chemists (AOAC), Washington, DC., USA.
  5. AOCS (American Oil Chemists Society). (1996). Official Methods and Recommended Practices of the American Oil Chemist’s Society, 5th Edn, Champaign.
  6. AOAC (Association of Official Analytical Chemists). (2000). Official Methods of Analysis, 17th Edn. AOAC International, Gaithersburg.
  7. Berhe Gebremedhn, M., Tessema, W., Gezimu Gebre, G., Tadesse Mawcha, K., & Kiros Assefa, M. (2017). Value chain analysis of sesame (Sesamum indicumL.) in Humera district, Tigray, Ethiopia. Cogent Food & Agriculture, 5(1), 1705741. DOI:
  8. Boutin, O., & Badens, E. (2009). Extraction from oleaginous seeds using supercritical CO2: Experimental design and products quality. Journal of Food Engineering, 92(4), 396–402. DOI:
  9. Çakaloğlu, B., Özyurt, H., & Ötleş, S. (2018). Cold press in oil extraction. A review. Ukrainian Food Journal, 7, 640–654. DOI:
  10. Chemat, F., Vian, M. A., Ravi, H. K., Khadhraoui, B., Hilali, S., Perino, S., & Tixier, A. S. F. (2019). Review of alternative solvents for green extraction of food and natural products: Panorama, principles, applications and prospects. Molecules, 24(16).
  11. DOI:
  12. Jeevan Kumar, S. P., Rajendra Prasad, S., Banerjee, R., Agarwal, D. K., Kulkarni, K. S., & Ramesh, K. v. (2017). Green solvents and technologies for oil extraction from oilseeds. Chemistry Central Journal, 11, 9. DOI:
  13. Katkade, M. B., Syed, H. M., Andhale, R. R., & Sontakke, M. D. (2018). Fatty acid profile and quality assessment of
  14. safflower (Carthamus tinctorius) oil. Journal of Pharmacognosy and Phytochemistry, 7(2), 3581–3585.
  15. Khoei, M., & Chekin, F. (2016). The ultrasound-assisted aqueous extraction of rice bran oil. Food Chemistry, 194, 503–507. DOI:
  16. Moreau, R. A., Johnston, D. B., Powell, M. J., & Hicks, K. B. (2004). A comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ. JAOCS, Journal of the American Oil Chemists’ Society, 81(11), 1071–1075. DOI:
  17. Negash, Y. A., Amare, D. E., Bitew, B. D., & Dagne, H. (2019). Assessment of quality of edible vegetable oils accessed in Gondar City, Northwest Ethiopia. BMC Research Notes, 12(1). DOI:
  18. Odoom, W., & Edusei, V. O. (2015). Evaluation of Saponification value, Iodine value and Insoluble impurities in Coconut Oils from Jomoro District in the Western Région of Ghana. Asian Journal of Agriculture and Food Sciences, 03, 2321 – 1571.
  19. Olaniyan, A. M., & Oje, K. (2011). Development of model equations for selecting optimum parameters for dry process of shea butter extraction. Journal of Cereals and Oilseeds, 2(4), 47–56.
  20. Pathak, N., Rai, A. K., Kumari, R., & Bhat, K. v. (2014). Value addition in sesame: A perspective on bioactive components for enhancing utility and profitability. Pharmacognosy Reviews, 8(16), 147–155. DOI: