PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
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Harshita Vashistha
Prashant Kumar
Sandeep Kumar
https://orcid.org/0009-0004-7943-5265
Prashant Kumar
Sandeep Kumar
Abstract
Global agriculture practices rely heavily on the application of fungicides, pesticides and synthetic fertilizers. Several bacterial strains were isolated from root nodules and screened for plant growth-promoting (PGP) activities. The selected isolates were characterized biochemically and identified by 16S rRNA sequencing. The ability of bacterial isolates to produce siderophores, phosphate solubilization, production of indole acetic acid (IAA) and other characteristics of plant growth promoting (PGP)activities were evaluated from the root nodules of a wild leguminous plant, Tephrosia purpurea (L.) Pers. Based on the results, the isolates were determined as Bacillus altitudinis and Pseudomonas azotoformans. The bacterial consortium consisting of B. altitudinis + P. azotoformans exhibited a substantial enhancement in both root length (35.55%), and shoot length (43.2%) when compared to the control. This study concludes that utilization of the beneficial traits of these non-rhizobial nodule endophytes bacteria improve nodulation biomass accumulation and plant vegetative growth parameters in T. purpurea. The present research has the potential to advance the adoption of eco-friendly agricultural practices and provide an alternative substitute for traditional chemical fertilizers.
How to Cite
Vashistha, H., Kumar, P., & Kumar, S. PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers. Environment Conservation Journal, 25(4). https://doi.org/10.36953/ECJ.27592833
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Keywords
Wild Legumes, PGPR, Biochemical Test, Vegetative Growth, Pot Trial
References
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Chen, W. F., Wang, E. T., Ji, Z. J., & Zhang, J. J. (2021). Recent development and new insight of diversification and symbiosis specificity of legume rhizobia: mechanism and application. Journal of Applied Microbiology, 131(2), 553-563. https://doi.org/10.1111/jam.14960
Chouhan, B., Tak, N., Bissa, G., Adhikari, D., Barik, S. K., Sprent, J. I. & Gehlot, H. S. (2022). Evolution of novel strains of Ensifer nodulating the invasive legume Leucaena leucocephala (Lam.) de Wit in different climatic regions of India through lateral gene transfer. FEMS Microbiology Ecology, 98(9), 1-19. https://doi.org/10.1093/femsec/fiac086
de Sousa, S. M., de Oliveira, C. A., Andrade, D. L., de Carvalho, C. G., Ribeiro, V. P., Pastina, M. M., & Gomes, E. A. (2021). Tropical Bacillus strains inoculation enhances maize root surface area, dry weight, nutrient uptake and grain yield. Journal of Plant Growth Regulation, 40(2), 867-877. https://doi.org/10.1007/s00344-020-10146-9
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Flores-Duarte, N. J., Caballero-Delgado, S., Pajuelo, E., Mateos-Naranjo, E., Redondo-Gomez, S., Navarro-Torre, S., & Rodríguez-Llorente, I. D. (2022). Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes. Frontiers in Microbiology, 13, 1005458. https://doi.org/10.3389/fmicb.2022.1005458
Glick, B. R. (2012). Plant growth-promoting bacteria: mechanisms and applications. Scientifica., 1-15. https://doi.org/10.6064/2012/963401
Gopalakrishnan, S., Sathya, A., Vijayabharathi, R., Varshney, R. K., Gowda, C. L. & Krishnamurthy, L. (2015). Plant growth promoting rhizobia: challenges and opportunities. 3 Biotechnology, 5(4), 355-377. https://doi.org/10.1007/s13205-014-0241-x
Gordon, S. A. & Weber, R. P. (1951). Colorimetric estimation of indoleacetic acid. Plant Physiology, 26(1), 192-195. https://doi.org/10.1104%2Fpp.26.1.192
Hakim, S., Imran, A. & Mirza, M. S. (2021). Phylogenetic diversity analysis reveals Bradyrhizobium yuanmingense and Ensifer aridi as major symbionts of mung bean (Vigna radiata L.) in Pakistan. Brazilian Journal of Microbiology, 52, 311-324.
Hyder, S., Rizvi, Z. F., los Santos-Villalobos, S. D., Santoyo, G., Gondal, A., Khalid, N., & Rani, A. (2023). Applications of plant growth-promoting rhizobacteria for increasing crop production and resilience. Journal of plant nutrition, 46(10), 2551-2580.
https://doi.org/10.1080/01904167.2022.2160742
Itelima, J. U., Bang, W. J., Onyimba, I. A., Sila, M. D. & Egbere, O. J. (2018). Bio-fertilizers as key player in enhancing soil fertility and crop productivity: A review. Direct Research Journal of the Science of Food and Agriculture, 6(3), 73-83.
Jensen, H. (1954). The azotobacteriaceae. Bacteriological Reviews, 18(4), 195-214.
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