Synergism of inert minerals and botanicals with spinosad: Co-toxicity against Callosobruchus maculatus in stored blackgram



Published Apr 20, 2024
Satwika Maharaj
Tirumalasetty Madhumathi
Bhimineni Ratna Kumari V. Prasanna Kumari


The investigation of “Inert Minerals and Botanicals Synergism with Spinosad: co-toxicity against Callosobruchus maculatus in Stored Blackgram” was performed in the Laboratory of the Department of Entomology, Agricultural College, Bapatla, during 2020-21. The inert minerals and botanicals were mixed with Spinosad and used for the bioassay. The data were recorded at 24, 48 and 72 hours after the release of the test insect, and probit analysis was performed to calculate the LD50. By using the LD50 of inert minerals and botanicals alone and in combination with spinosad, the co-toxicity coefficient was calculated. Among all the treatments, the highest co-toxicity coefficient (433.33) was recorded for diatomaceous earth, indicating increased efficacy and synergism between diatomaceous earth and Spinosad.

How to Cite

Maharaj, S., Madhumathi, T., Kumari, B. R., & Kumari, V. P. (2024). Synergism of inert minerals and botanicals with spinosad: Co-toxicity against Callosobruchus maculatus in stored blackgram. Environment Conservation Journal.


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Bioassay, Co-toxicity coefficient, Diatomaceous earth, LD50, Probit analysis

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