@article{Singh_Nikunjkumar_Saxena_Ramakrisnan_2021, title={Immunostimulatory potential of Nigella sativa seeds extract on chicken macrophage function }, volume={22}, url={https://journal.environcj.in/index.php/ecj/article/view/854}, DOI={10.36953/ECJ.2021.22350}, abstractNote={<p><strong>In present study, the effect of <em>Nigella sativa</em> seeds extract on the chicken peripheral blood mononuclear cells (PBMCs) was investigated. The chicken PBMCs were stimulated with two different doses (125?g/ml; 250?g/ml) of <em>Nigella sativa</em> seeds extract and the cells were harvested at different time points till 48h post-stimulation for analysis of iNOS gene expression by quantitative PCR and nitric oxide (NO) estimation at 24 and 48h post-stimulation. High dose (250?g/ml) of <em>Nigella sativa</em> seed extract showed remarkable induction of iNOS transcripts expression and NO production at 48h post-stimulation, which were 12.8040±1.03347 folds and 5.7089±.64535µM respectively. The results indicated the immunostimulatory potential of the <em>Nigella sativa</em> seed extract on the chicken PBMCs.</strong></p>}, number={3}, journal={Environment Conservation Journal}, author={Singh, Yogendra and Nikunjkumar, Patel and Saxena, Beenam and Ramakrisnan , Saravanan}, year={2021}, month={Dec.}, pages={433–439} }