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September 13, 2021
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October 17, 2021
Published
December 19, 2021
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Abstract

In present study, the effect of Nigella sativa seeds extract on the chicken peripheral blood mononuclear cells (PBMCs) was investigated. The chicken PBMCs were stimulated with two different doses (125?g/ml; 250?g/ml) of Nigella sativa seeds extract and the cells were harvested at different time points till 48h post-stimulation for analysis of iNOS gene expression by quantitative PCR and nitric oxide (NO) estimation at 24 and 48h post-stimulation. High dose (250?g/ml) of Nigella sativa seed extract showed remarkable induction of iNOS transcripts expression and NO production at 48h post-stimulation, which were 12.8040±1.03347 folds and 5.7089±.64535µM respectively. The results indicated the immunostimulatory potential of the Nigella sativa seed extract on the chicken PBMCs.

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iNOS Macrophages Nitric oxide PBMCs

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Singh, Y. . ., Nikunjkumar, P. . ., Saxena, B., & Ramakrisnan , S. . (2021). Immunostimulatory potential of Nigella sativa seeds extract on chicken macrophage function . Environment Conservation Journal, 22(3), 433–439. https://doi.org/10.36953/ECJ.2021.22350
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References

  1. Al Mahmud, H., Seo, H., Kim, S., Islam, M. I., Nam, K. W., Cho, H. D., & Song, H. Y. (2017). Thymoquinone (TQ) inhibits the replication of intracellular Mycobacterium tuberculosis in macrophages and modulates nitric oxide production. BMC complementary and alternative medicine, 17(1), 1-8. DOI: https://doi.org/10.1186/s12906-017-1786-0
  2. Bahrami, M., Ghazavi, A., Ganji, A., & Mosayebi, G. (2021). Anti-Inflammatory Activity of S. Marianum and Nigella sativa Extracts on Macrophages. Reports of Biochemistry & Molecular Biology, 10(2), 288. DOI: https://doi.org/10.52547/rbmb.10.2.288
  3. Bashir, K., Kappala, D., Singh, Y., Dar, J. A., Mariappan, A. K., Kumar, A., & Ramakrishnan, S. (2019). Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken. Scientific reports, 9(1), 1-16. DOI: https://doi.org/10.1038/s41598-019-44578-5
  4. Berzi, A., Varga, N., Sattin, S., Antonazzo, P., Biasin, M., Cetin, I., & Clerici, M. (2014). Pseudo-mannosylated DC-SIGN ligands as potential adjuvants for HIV vaccines. Viruses, 6(2), 391-403. DOI: https://doi.org/10.3390/v6020391
  5. Boeglin, E., Smulski, C. R., Brun, S., Milosevic, S., Schneider, P. & Fournel, S. (2011). Toll-like receptor agonists synergize with CD40L to induce either proliferation or plasma cell differentiation of mouse B cells. PLoS One, 6(10), e25542. DOI: https://doi.org/10.1371/journal.pone.0025542
  6. Carnagarin, R., Matthews, V., Zaldivia, M. T., Peter, K., & Schlaich, M. P. (2019). The bidirectional interaction between the sympathetic nervous system and immune mechanisms in the pathogenesis of hypertension. British journal of pharmacology, 176(12), 1839-1852. DOI: https://doi.org/10.1111/bph.14481
  7. Chen, S. M., Shen, H., Zhang, T., Huang, X., Liu, X. Q., Guo, S. Y., & An, M. M. (2017). Dectin-1 plays an important role in host defense against systemic Candida glabrata infection. Virulence, 8(8), 1643-1656. DOI: https://doi.org/10.1080/21505594.2017.1346756
  8. Darmawan, W., Farida, R., & Redjeki, S. (2019). The effect of nigella sativa (Black cumin) seed extract on Candida albicans viability. International Journal of Applied Pharmaceutics, 11, 88-91. DOI: https://doi.org/10.22159/ijap.2019.v11s1.199
  9. Elmowalid, G., Amar, A. M., & Ahmad, A. A. M. (2013). Nigella sativa seed extract: 1. Enhancement of sheep macrophage immune functions in vitro. Research in veterinary science, 95(2), 437-443. DOI: https://doi.org/10.1016/j.rvsc.2013.02.015
  10. Gholamnezhad, Z., Rafatpanah, H., Sadeghnia, H. R., & Boskabady, M. H. (2015). Immunomodulatory and cytotoxic effects of Nigella sativa and thymoquinone on rat splenocytes. Food and Chemical Toxicology, 86, 72-80. DOI: https://doi.org/10.1016/j.fct.2015.08.028
  11. Gordon, S. (2016). Phagocytosis: an immunobiologic process. Immunity, 44(3), 463-475. DOI: https://doi.org/10.1016/j.immuni.2016.02.026
  12. Hwang, J. R., Cartron, A. M., & Khachemoune, A. (2021). A review of Nigella sativa plant?based therapy in dermatology. International Journal of Dermatology. DOI: https://doi.org/10.1111/ijd.15615
  13. Khan, J., Noboru, N., Young, A., & Thomas, D. (2017). Pro and anti-inflammatory cytokine levels (TNF-?, IL-1?, IL-6 and IL-10) in rat model of neuroma. Pathophysiology, 24(3), 155-159. DOI: https://doi.org/10.1016/j.pathophys.2017.04.001
  14. Kiboneka, A. (2021). Principals of innate and adaptive immunity. Immunity to microbes & fundamental concepts in immunology. World Journal of Advanced Research and Reviews, 10(3), 188-197. DOI: https://doi.org/10.30574/wjarr.2021.10.3.0271
  15. Kishik, S., Nagati, I., El Hayawan, I., Ali, I., Fawzy, M., & Ali, H. (2020). Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment. Parasitologists United Journal, 13(3), 172-178. DOI: https://doi.org/10.21608/PUJ.2020.41053.1086
  16. Koshak, A. E., Yousif, N. M., Fiebich, B. L., Koshak, E. A., & Heinrich, M. (2018). Comparative immunomodulatory activity of Nigella sativa L. preparations on proinflammatory mediators: A focus on asthma. Frontiers in pharmacology, 9, 1075. DOI: https://doi.org/10.3389/fphar.2018.01075
  17. Kuo, S. M., Chen, C. J., Chang, S. C., Liu, T. J., Chen, Y. H., Huang, S. Y., & Shih, S. R. (2017). Inhibition of avian influenza A virus replication in human cells by host restriction factor TUFM is correlated with autophagy. MBio, 8(3), e00481-17. DOI: https://doi.org/10.1128/mBio.00481-17
  18. Manoharan, N., Jayamurali, D., Parasuraman, R., & Govindarajulu, S. N. (2021). Phytochemical composition, therapeutical and pharmacological potential of Nigella sativa: a review. Tradit Med Res, 6(4), 32. DOI: https://doi.org/10.53388/TMR20210118216
  19. Matsubara, K., Higaki, T., Matsubara, Y., & Nawa, A. (2015). Nitric oxide and reactive oxygen species in the pathogenesis of preeclampsia. International journal of molecular sciences, 16(3), 4600-4614. DOI: https://doi.org/10.3390/ijms16034600
  20. Mehkri, S., Chandrasagar, K., Ashok, G. and Bopanna, K.2021. Evaluation of In Vitro phagocytic property of macrophages in presence of Thymopure™ (Nigella Sativa) oil. International Journal of Biology Research, 6(2):18-21.
  21. Miliani, M., Nouar, M., Paris, O., Lefranc, G., Mennechet, F., & Aribi, M. (2018). Thymoquinone potently enhances the activities of classically activated macrophages pulsed with necrotic jurkat cell lysates and the production of antitumor Th1-/M1-related cytokines. Journal of Interferon & Cytokine Research, 38(12), 539-551. DOI: https://doi.org/10.1089/jir.2018.0010
  22. Pfaffl, M. W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic acids research, 29(9), e45-e45. DOI: https://doi.org/10.1093/nar/29.9.e45
  23. Ren, D., Lin, D., Alim, A., Zheng, Q., & Yang, X. (2017). Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-?B signaling pathways in RAW264. 7 cells. Food & function, 8(3), 1299-1312. DOI: https://doi.org/10.1039/C6FO01699E
  24. Swamy, S. M. K., & Tan, B. K. H. (2000). Cytotoxic and immunopotentiating effects of ethanolic extract of Nigella sativa L. seeds. Journal of ethnopharmacology, 70(1), 1-7. DOI: https://doi.org/10.1016/S0378-8741(98)00241-4
  25. Tejero, J., Shiva, S., & Gladwin, M. T. (2019). Sources of vascular nitric oxide and reactive oxygen species and their regulation. Physiological reviews, 99(1), 311-379. DOI: https://doi.org/10.1152/physrev.00036.2017
  26. Tripathi, Y. B., Chaturvedi, A. P., & Pandey, N. (2012). Effect of Nigella sativa seeds extracts on iNOS through antioxidant potential only: crude/total extract as molecular therapy drug.
  27. Wang, L. X., Zhang, S. X., Wu, H. J., Rong, X. L., & Guo, J. (2019). M2b macrophage polarization and its roles in diseases. Journal of leukocyte biology, 106(2), 345-358. DOI: https://doi.org/10.1002/JLB.3RU1018-378RR
  28. Yimer, E. M., Tuem, K. B., Karim, A., Ur-Rehman, N., & Anwar, F. (2019). Nigella sativa L.(black cumin): a promising natural remedy for wide range of illnesses. Evidence-Based Complementary and Alternative Medicine, 2019. DOI: https://doi.org/10.1155/2019/1528635
  29. Zhang, B., Gan, L., Shahid, M. S., Lv, Z., Fan, H., Liu, D., & Guo, Y. (2019). In vivo and in vitro protective effect of arginine against intestinal inflammatory response induced by Clostridium perfringens in broiler chickens. Journal of animal science and biotechnology, 10(1), 1-14. DOI: https://doi.org/10.1186/s40104-019-0371-4