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In present study, the effect of Nigella sativa seeds extract on the chicken peripheral blood mononuclear cells (PBMCs) was investigated. The chicken PBMCs were stimulated with two different doses (125?g/ml; 250?g/ml) of Nigella sativa seeds extract and the cells were harvested at different time points till 48h post-stimulation for analysis of iNOS gene expression by quantitative PCR and nitric oxide (NO) estimation at 24 and 48h post-stimulation. High dose (250?g/ml) of Nigella sativa seed extract showed remarkable induction of iNOS transcripts expression and NO production at 48h post-stimulation, which were 12.8040±1.03347 folds and 5.7089±.64535µM respectively. The results indicated the immunostimulatory potential of the Nigella sativa seed extract on the chicken PBMCs.


iNOS Macrophages Nitric oxide PBMCs

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Singh, Y. . ., Nikunjkumar, P. . ., Saxena, B., & Ramakrisnan , S. . (2021). Immunostimulatory potential of Nigella sativa seeds extract on chicken macrophage function . Environment Conservation Journal, 22(3), 433–439.


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