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Abstract

Abstract: Ascorbic acid is one of the important tool to indicate the alterations induced by chemicals and pollutants. Ascorbic acid, being important constituent in cellular metabolism, the interactions of the biomolecules gives proper idea of toxicant stress and its effect. In the present investigation the freshwater bivalve Lamellidens marginalis were exposed to chronic dose of lambda-cyalothrin (0.75 PPM LC50/10 values) alone and in combination with 50mg/L L-ascorbic acid for 21 days respectively. Percent protein and ascorbic acid contents in the mantle, foot, gills, digestive glands, gonad and whole soft body of bivalve, Lamellidens marginalis on lambda-cyalothrin intoxication and in combination with 50mg/L L-ascorbic acid were observed. Protein and ascorbic acid content in all soft body tissue of lambda-cyalothrin exposed bivalve, Lamellidens marginalis showed remarkable decrease as compared to control. The maximum protein and ascorbic acid content was observed in foot and lowest in digestive gland. Animal exposed to lambda-cyalothrin intoxication in combination with 50 mg/L of L-ascorbic acid showed considerable reduction in the depletion of protein and ascorbic acid levels. Fast recovery of percent protein and ascorbic acid contents was observed in presence of L-ascorbic acid than the recovery in the normal freshwater. This study indicates the protective and curative property of the L-ascorbic acid against lambda-cyalothrin induced damage.


Key Words: Lamellidens marginalis, lambda-cyalothrin, protein and L-ascorbic acid

Keywords

Ascorbic acid Lamellidens marginalis Lambda-cyhalothrin protein

Article Details

How to Cite
Goswami, D. B. ., & Bhalla, R. (2021). Ameliorating effect of L-Ascorbate on protein and ascorbic acid content in different tissues of freshwater bivalve Lamellidens marginallis on exposure to lambda-cyalothrin. Environment Conservation Journal, 22(1&2), 159–166. https://doi.org/10.36953/ECJ.2021.221222

References

  1. Ahmad, I., Mohmood, I., Ceolho, J. P., Pacheco, M., Santos, M. A., Duarte, A. C. Pereira, E. 2012. Role of non-enzymatic antioxidants on the bivalves' adaptation to environmental mercury: Organ-specificities and age effect in Scrobicularia plana inhabiting a contaminated lagoon. Environmental pollution, 1(13): 218-225.
  2. Bhalla, R. 2019. Methomyl and Lambda-cyhalothrin induced alterations in the protein content and recovery due to L-ascorbic acid in different tissues of the freshwater bivalve, Lamellidens marginalis (Lamarck). International Journal of Global Science Research, 6 (1): 653-662.
  3. Bindhumol, V., Chitra, K. C., Mathur, P. P. 2003. A induces reactive oxygen species generation in the liver of male rats. Toxicology, 188: 117-124.
  4. Duarte, T. L. and Lunec, J. 2005. When is an antioxidant not an antioxidant? A review of novel actions and reactions of vitamin C. Free Radical Research, 39(7): 671-686.
  5. Fadime Ery?lmaz Pehlivan, 2017. Vitamin C: An Antioxidant Agent http://dx.doi.org/10.5772/intechopen.69660
  6. Finney, D. J. 1971. Probit Analysis (3rd edition). Cambridge University Press, Cambridge, UK.
  7. Jayakumar, P., Jothivel, N. and Paul, V. 2008. Heavy metals induced alterations in the acid phosphates activity in the edible freshwater mussel Lamellidens marginalis (Lamarck). The International Journal Toxicology, 5(2): DOI: 10.5580/2559.
  8. Kamble, V. S., Gavhane, U. V. and Rao, K. R. 2011. Impact of Organochlorine and Organophosphorus pesticides on ascorbic acid content in fresh water lamellibranch mollusc Lamellidens corrianus during monsoon season. Indian Journal of Fundamental and Applied Life Sciences, 1(2): 146-149.
  9. Lowry, O. M, Rosenbroughty, N. J., Farr A. L., Randall, R. F. 1951. Protein estimation with folin phenol reagent. Journal of Biochemistry, 193: 265-275.
  10. Mahajan, S. S., Zhambare, S. P., Phanse, M. P. 2015. Cure of arsenic induced alterations in the protein levels of an experimental model, Lamellidens marginalis by L-Ascorbic acid. BionanoFrontier, 8(3): 110- 113.
  11. Malathi, S. and Thippeswamy, S. 2013. The Proximal and mineral compositions of fresh water mussels Parreysia corrugata (Mullar,1774) from Tunga river in the western ghats, India. G.J.B.A. H.S. 2(3):165-170.
  12. Patil, A. G. 2011. Protein changes in different tissues of freshwater bivalve Parreysia cylindrica after exposed to indoxacarb. Recent Research in Science and Technology, 3(3): 140-142.
  13. Rahane, B. and Bhalla, R. 2018. Toxicant stress on protein and ascorbic acid contents in different tissues of freshwater bivalve Parreysia cylindrica from different reservoirs of Nashik District. India. Eco. Env. & Cons, 24 (2): 856-860.
  14. Rahane, B. and Bhalla, R.2019. Biochemical alterations due to heavy metals in different tissues of freshwater bivalve species at Chankapur reservoirs of Nasik district. (M.S). Journal of Environment and Biological sciences, 33 (1): 137-142.
  15. Roe, J. H. 1967. In: Methods of Biochemical analysis Vol. 5, Ed. Blick, Interscience, New York: 44.
  16. Tambe, R. S., Pulate, P. D. 2015. Protective role of l–ascorbic acid on dichlorovos induced alterations in the protein contents of the fresh water bivalve, Parreysia cylindrica, Indian Journal of Applied Research, 5(9): 288-291.
  17. Waykar and Tambe, 2014: Curative role of l-ascorbic acid on dicofol induced alterations of the protein levels in the freshwater bivalve, Parreysia cylindrica. Indian Journal of Applied Research Volume, 4 (50): 641-644.