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Abstract

Little is known about rate of photosynthesis, transpiration, stomatal conductance, intrinsic water use efficiency, endogenous phytohormones (Gibberellic acid and Indole acetic acid) concomitant with leaf chlorophyll content during flowering and fruiting stages in pomegranate. As a result, a study was conducted to determine the function of various chemicals, such as Nitrobenzene (NB) at 1.0, 1.5, and 2.0 ml plant-1, Cycocel (CCC) at 500, 1000, and 1500 ppm plant-1, Uracil 25 and 50 ppm plant-1, CCC1000 ppm + Uracil 25 ppm plant-1, CCC 1500 ppm + Uracil 50 ppm plant-1, along with control at ICAR - Indian Institute of Horticultural Research (IIHR), Bengaluru, during the flowering and fruit set stages during two seasons, namely ambe bahar (January–February 2016) and hastha bahar (September–October 2016-17). Applications of Uracil 50 ppm plant-1, Uracil 25 ppm plant-1 and nitrobenzene 1.5 ml plant-1 at flowering stage resulted in highest rate of photosynthesis (16.48 µmol m-2 s-1), transpiration (7.69 mmol m-2 s-1) and stomatal conductance (0.44 mol m-2 s-1). Foliar spray of  Nitrobenzene 1.5 ml plant-1, cycocel 1000 ppm plant-1 + Uracil 25 ppm plant-1 in conjunction with application of nitrobenzene 1.0 ml plant-1 registered highest rate of photosynthesis (12.55 µmol m-2 s-1), transpiration (6.19 mmol m-2 s-1) and stomatal conductance (0.19 mol m-2 s-1) at fruit set stage. Application of Uracil 50 ppm plant-1 resulted in higher levels of endogenous IAA content (394.3 ng g-1 FW and 885.3 ng g-1 FW), lower levels of GA3 content (110.7 ng g-1 FW and 144.0 ng g-1 FW), and highest total chlorophyll content (3.14 mg g-1 and 1.99 mg g-1) in the leaves at both flowering and fruit set stages. With application of cycocel 1500 ppm plant-1 at flowering and fruit set stages, highest photosynthetic rate (17.67 µmol m-2 s-1 and 15.71 µmol m-2 s-1), transpiration rate (7.68 mmol m-2 s-1 and 6.13 mmol m-2 s-1) and higher levels of endogenous IAA (946.7 ng g-1 FW and 633.0 ng g-1 FW) were registered respectively. Following application of cycocel 1500 ppm plant-1 and 500 ppm plant?1 at both flowering and fruit set stages, lower endogenous GA3 (163.3 ng g-1 FW and 276.0 ng g-1 FW) were observed. At flowering stage, cycocel 1000 ppm plant-1 + Uracil 25 ppm plant-1 recorded the highest total chlorophyll content (2.35 mg g-1). During flowering period, cycocel 1500 ppm plant-1 application resulted in the highest stomatal conductance (0.57 mol m-2 s-1). Application of cycocel 1500 ppm plant-1 + Uracil 50 ppm plant-1 resulted in the highest stomatal conductance (0.38 mol m-2 s-1) at fruit set stage.


 

Keywords

Bhagwa Physiological Parameters Plant Growth Regulators Pomegranate

Article Details

How to Cite
S., F., Murthy, B., Reddy, M. ., Upreti, K. K. ., Satisha, J. ., Laxman, R. H. ., Srinivasulu, B. ., & Reddy, S. S. . (2021). Effect of different chemical and seasons on gas exchange parameters, phytohormones, and chlorophyll content in tissue culture plants of Pomegranate (Punica granatum L.) cv. Bhagwa . Environment Conservation Journal, 22(1&2), 127–135. https://doi.org/10.36953/ECJ.2021.221217

References

  1. Azcon –Bieto, J. 1983. Inhibition of photosynthesis by carbohydrates in wheat leaves. Plant Physiology, 73: 681-686.
  2. Arun Kumar, Sharma, N. and Gopal Singh. 2016. Effect of paclobutrazol and cycocel on growth, flowering and physiological characteristics of olive cv. Pendulino. Plant Archives, 16: 110-114.
  3. Arun Kumar, Singh, R.P. and Sharma, N. 2017. Influence of paclobutrazol and cycocel on growth, fruitfulness and physiological characteristics of olive (Olea europea L.). Plant Archives, 17: 83-88.
  4. Bankar, G.J. and Prasad, R.N. 1992. Performance of important pomegranate cultivars in arid region. Annals of Arid Zone. 31(3): 181-183.
  5. Blanco, A, Lowery, M, Montero, M, Mora-urpi, J. and Rojas pejibaye, M. El 1997. su uso en la alimentación humana. San Jose: Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud. 1-7.
  6. Caemmerer Von S. and Farquhar, G.D. 1981. Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta. 153: 376-387.
  7. Chung, J., Park, K. Y., Kim, Y. H., Jee, S. and Jaechul, K. 1999. Effect of growth retardants on the growth of Bletellastriata In Vitro. Journal of Korean Society of Horticultural Sciences. 40: 485-488.
  8. Jadhav, V.T. and Sharma, J. 2007. Pomegranate cultivation is promising. Indian Horticulture. 30-31.
  9. Jain, M.C. and Dashora, L.K. 2007. Growth, flowering, fruiting and yield of guava (Psidium guajava L.) cv. SARDAR as influenced by various plant growth regulators. International Journal of Agricultural Sciences. 3(1): 4-7.
  10. Jarvis, P.G. and Morison, J.I.L. 1981. The control of transpiration and photosynthesis by the stomata. In Stomatal Physiology. Eds. P.G. Jarvis and T.A. Mansfield. Cambridge University Press, Cambridge, U.K., PP 247-279.
  11. Jones, H.G. 1992. Stomatal control of photosynthesis and transpiration. Journal of experimental Botany. 49: 387-398.
  12. Kelen, M., Demiralay, E.C, Sen, S. and Ozkan, G. 2004. Separation of abscisic acid, indole-3-acetic acid, gibberellic acid in 99 R (Vitis berlandieri × Vitis 17 rupestris) and rose oil (Rosa damascena Mill.) by Reversed Phase Liquid Chromatography. Turkish Journal of Chemistry. 28: 603-610.
  13. Lenz, F. and Kuntzel, U. 1974. Carbohydrate content of citrus as affected by fruit load. – Gartenbauwissenschaft. 39: 99-101.
  14. Lewis, J.D., Wang, X.Z., Griffin, K.L. and Tissue, D.T. 2002. Effects of age and ontogent on photosynthetic responses of a determinate annual plant to elevated CO2 concentrations. Plant Cell Environment. 25: 359-368.
  15. Lichtenthaler, H.K. and Buschmann, C. 2001. Chlorophylls and Carotenoids. Pigments of photosynthetic biomembranes. Journal of enzyme and chemistry. 148:350-382.
  16. Liu, X., Robinson, P.W., Madore, M.A., Witney, G.W. and Arpaia, M.L. 1999. ‘Hass’ avocado carbohydrate fluctuations. I. Growth and phenology. Journal of American Society of Horticultural Sciences. 124: 671-675.
  17. Masarovicova, E., and Novara, J. 1994. Influence of fruit load on CO2 exchange, water uptake and biomass of apple trees. Gartenbauwissenchaft. 59: 132-138.
  18. Monselise, S.P. and Lenz, F. 1980. Effect of fruit load on stomatal resistance, specific leaf weight, and water content of apple leaves. Gartenbauwissenschaft. 45: 188-191.
  19. Monteith, J.L. and Unsworth, M.H. 1990. Principles of environmental physics, 2nd Edn. Edward Arnold, London, 291p.
  20. Moore, B.D., Cheng, S.H., Sims, D. and Steemann, J.R. 1999. The biochemical and molecular basis for photosynthetic acclimation to elevated atmospheric CO2. Plant Cell Environment. 22: 567-582.
  21. Pal, R.K. and Babu, K.D. 2014. Postharvest management and total utilization of pomegranate (Punica granatum L.). (In) Souvenir – National seminar – cum – exhibition on Pomegranate for nutrition, livelihood security and entrepreneurship development, 05-07 December 2014, ICAR – NRC on Pomegranate, Solapur, pp 252 – 261.
  22. Ramteke, S.D. and Somkumar, R.G. 2005. Effect of cycocel sprays on growth and yield of Tas – A – Ganesh grapes grafted on Dogridge Rootstock. Karnataka Journal of Agricultural Sciences. 18(1): 18-20.
  23. Rogers, A., Allen, D.J., and Davey. 2004. Leaf photosynthesis and carbohydrate dynamics of soybeans grown through out their life cycle under free air carbon dioxide enrinchment. Plant Cell Environment. 27: 449-458.
  24. Rook, F., Hadingham, S.A., Li, Y. and Bevan, M.W. 2006. Sugar and ABA response pathways and the control of gene expression. Plant Cell Environment. 29: 426-434.
  25. Schroeder, J., Kwak, J. M. and Allen, G.J. 2001. Guard cell Abscicic acid signaling and engineering drought hardiness in plants. Nature. 410: 327.330.
  26. Sevda, S.B. and Rodrigues, L. 2011. The making of pomegranate wine using yeast immobilized sodium alginate. African Journal of Food Science. 5: 299-304.
  27. Sharma, K.K. and Nav Prem, S. 2002. Effect of mechanical deblossoming on fruit development in pomegranate (Punica granatum L.). Journal of Research Punjab Agricultural University. 39: 368 – 372.
  28. Srilatha, V., Reddy, Y.T.N., Upreti, K. K. and Jagannath, S. 2015. Pruning and paclobutrazol induced vigour, flowering and hormonal changes in mango (Mangifera indica L.). The Bioscan. 10: 161-166.
  29. Soumya, P.R., Pramod kumar and Madan Pal. 2017. Paclobutrazol : a novel plant growth regulator and multi-stress ameliorant. Indian Journal of Plant Physiology. 22(3): 267-278.
  30. Terashima Ichiro and John Evans, R. 1988. Effects of light and nitrogen nutrition on the organisation of the photosynthetic approach in Spinach. Plant and Cell Physiology. 29: 143-155.