Main Article Content
Abstract
The ability to degrade the diesel by bacterial species (Micrococcus spp., Staphylococcus spp., and Celibiosococcus spp.) as a sole source of hydrocarbon was studied in the present work. In laboratory by identifying and assessing the potential of hydrocarbon degrader by micro titer plate method, the best diesel degrading bacteria was isolated. The similarity trait among the 25 bacterial isolates was also assessed. All the bacterial species utilized the hydrocarbons as sole carbon and energy sources showing increases in cell number and optical density with decreases in pH of the culture media. This study shown that Micrococcus spp., has maximum ability to degrade diesel. The banding pattern of RAPD showed that all the isolates share some similarity between them RAPD was chosen as it has been shown to be effective sub typing method for several other species.
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References
- American Public Health Association (APHA) 1985. Standard methods for the Examination of water and wastewater, 16th ed., American Public Health Association, Washington, DC.
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- Bryant, T. N. 2003. Probablistic Identification of bacteria, PIB computer kit, medical statistics and computing University Southampton, S094 XYUK.
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- Colwell, R. R. and J. D. Walker 1977. Ecological aspects of microbial degradation of oil in the marine environment. Crit. Rev. Microbiol: Article in press.
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- Gram, C. 1884. The differential staining of Schzomycetes in tissue sections and in dried preparations. Forts Chritee. Der. Medicin. 2: 185-189.
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- Mac Faddin, F. J. 1980. Biochemical tests for identification of medical bacteria. Williams and Wilkins Co. Baltsimore Vol. 1, USA.
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- Mehdi, H. and E. Giti 2008. Investigation of alkane biodegradation using the microtiter Plate method and correlation between biofilm formation, biosurfactant production and crude oil biodegradation. Int. Biodeterioration and biodegradation. Article in press.
- Muyzer, G. and K. Smalla 1999. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis in microbial ecology. Antonie Van Leeuwenhock. 73: 121-141.
- Nilanjana Das, Preethy Chandran 2010. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview, 2011: 1-13.
- Oboh, B. O., M. O. Ilori, J. O. Akinyemi, S. A. Adebusoye 2006. Hydrocarbon degrading potentials of bacteria isolated from a Nigerian Bitumen (Tarsand) deposit. Nature and Science. 4(3): 51-57.
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- Riser-Roberts, E. 1992. Bioremediation of petroleum contaminated sites. Boca Raton (FL): CRC press INC.
- Roberts, M. A. and D. L. Crawford 2000. Use of randomly amplified poliymorphic DNA as a means of developing Genus- and strain-specific Streptomyces DNA probes. Appl and Environ. Microbiol. 66(6): 2555-2564.
- Sebiomo A, Bankole SA, Awosanya AO 2010. Determination of the ability of microorganisms isolated from mechanic soil to utilize lubricating oil as carbon source. Afr. J. Microbiol. Res., 4: 2257-2264.
- Sharma, N., Y. Sudarsan, R. Sharma, G. Singh 2008. RAPD analysis of soil microbial diversity in western Rajasthan. Current Sci. 94(8): 1058-1061.
- Van Soolingen, D. and P. W. M. Hermans 1995. Epidemiology of tuberculosis by DNA finger printing. European Respir. J. 20(8): 649-656.
- Vinothini, C., S. Sudhakar, R. Ravikumar 2015. Biodegradation of petroleum and crude oil by Pseudomona putida and Bacillus cereus. Int. J. curr. Microbiol. App. Sci. 4(1): 318-329.
- Whyte, L. G., J. Hawari, E. Zhou, L. Bourbonniere, W. E. Inniss, C. W. Greer 1998. Biodegradation of variable chain-Length-Alkanes at low temperatures by a Psychrotrophic Rhodococcus sp. Appl. Environ. Microbiol. 64(7): 2578- 2584.
References
American Public Health Association (APHA) 1985. Standard methods for the Examination of water and wastewater, 16th ed., American Public Health Association, Washington, DC.
Bento, F. M., F. A. O. Camargo, B. C. Okeke, W. T. FrangKenberger 2005. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresour. Technol. 96: 1049-1055.
Bryant, T. N. 2003. Probablistic Identification of bacteria, PIB computer kit, medical statistics and computing University Southampton, S094 XYUK.
Calomiris, J. J., B. Austin, J. D. Walker, R. R. Colwell 1976. Enrichment for estuarine petroleum degrading bacteria using liquid and solid media. Journ. Appl. Bacteriol. 42: 135-144.
Chauhan, S. and R. S. Chaurasiya 2012. Bioremediation of diesel contaminated soil through microbial flora. Env. Cons. Jour. 13(1&2): 111-114.
Colwell, R. R. and J. D. Walker 1977. Ecological aspects of microbial degradation of oil in the marine environment. Crit. Rev. Microbiol: Article in press.
Das, K. and A. K. Mukherjee 2007. Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petrolum-oil contaminated soil from North-East India. Bioresour. Technol. 98: 1339-1345.
El-Hanafy, A. A., H. E. Abd-Elsalam, E. E. Hafez 2007. Fingerprinting for the Lignin degrading bacteria from the soil. Journ. Of Appl. Sci. Res. 3(6): 470-475.
Gram, C. 1884. The differential staining of Schzomycetes in tissue sections and in dried preparations. Forts Chritee. Der. Medicin. 2: 185-189.
Heller, H. and K. Smalla 1997. Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis for studying soil microbial communities. In modern soil microbiology. Van Elsas, JD: J. T. Trevors, E. M. H. Wellington (eds.). Marcel Dekker, Inc., Newyork: 353-373.
Johnsen, K., C. S. Jacobsen, V. Torsvik, J. Sorenson 2001. Pesticide effects on bacterial diversity in agricultural soils- A review Biology and fertility of soils. 33: 443-453.
Krieg, R. N. and G. Holt 1984. In: Bergey’s Manual of Systematic Bacteriology, William and Witkins Co. Baltimore: 1.
Mac Faddin, F. J. 1980. Biochemical tests for identification of medical bacteria. Williams and Wilkins Co. Baltsimore Vol. 1, USA.
Margesin, R. and F. Schinner 2001. Bioremediation (natural attenuation and biostimulation) of diesel-oil-contaminated soil in an alpine glacier skiing area. Appl. Environ. Microbiol. 67(7): 3127-3133.
Marin, M., A. Pedregosa, S. Rios, F. Laborda 1996. Study of factors influencing the degradation of heating oil by Acinetobacter calcoaceticus MM5. Int. Biodeterior. Biodegradation. 38: 69–75.
Mehdi, H. and E. Giti 2008. Investigation of alkane biodegradation using the microtiter Plate method and correlation between biofilm formation, biosurfactant production and crude oil biodegradation. Int. Biodeterioration and biodegradation. Article in press.
Muyzer, G. and K. Smalla 1999. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis in microbial ecology. Antonie Van Leeuwenhock. 73: 121-141.
Nilanjana Das, Preethy Chandran 2010. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview, 2011: 1-13.
Oboh, B. O., M. O. Ilori, J. O. Akinyemi, S. A. Adebusoye 2006. Hydrocarbon degrading potentials of bacteria isolated from a Nigerian Bitumen (Tarsand) deposit. Nature and Science. 4(3): 51-57.
Prince, R. C. 1993. Petroleum spill bioremediation in marine environments. Critical reviews in microbiology. 19: 217-242.
Rahman, K. S. M., J. T. Rahman, Y. kourkoutas, I. Petsas, R. Marchant, I. M. Banat, 2003. Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients. Bioresour. Technol. 90: 159-168.
Riser-Roberts, E. 1992. Bioremediation of petroleum contaminated sites. Boca Raton (FL): CRC press INC.
Roberts, M. A. and D. L. Crawford 2000. Use of randomly amplified poliymorphic DNA as a means of developing Genus- and strain-specific Streptomyces DNA probes. Appl and Environ. Microbiol. 66(6): 2555-2564.
Sebiomo A, Bankole SA, Awosanya AO 2010. Determination of the ability of microorganisms isolated from mechanic soil to utilize lubricating oil as carbon source. Afr. J. Microbiol. Res., 4: 2257-2264.
Sharma, N., Y. Sudarsan, R. Sharma, G. Singh 2008. RAPD analysis of soil microbial diversity in western Rajasthan. Current Sci. 94(8): 1058-1061.
Van Soolingen, D. and P. W. M. Hermans 1995. Epidemiology of tuberculosis by DNA finger printing. European Respir. J. 20(8): 649-656.
Vinothini, C., S. Sudhakar, R. Ravikumar 2015. Biodegradation of petroleum and crude oil by Pseudomona putida and Bacillus cereus. Int. J. curr. Microbiol. App. Sci. 4(1): 318-329.
Whyte, L. G., J. Hawari, E. Zhou, L. Bourbonniere, W. E. Inniss, C. W. Greer 1998. Biodegradation of variable chain-Length-Alkanes at low temperatures by a Psychrotrophic Rhodococcus sp. Appl. Environ. Microbiol. 64(7): 2578- 2584.