Main Article Content

Abstract

Heavy metal pollution of soil, water bodies and air is one of the alarming issues in all over the world. The present investigation mainly deals with the isolation, identification and screening of the metal sorption capacity of indigenous fungal strains isolated from iron mining area, Barbil, Odisha, India. Major fungal genera observed were Aspergillus sp., Rhizopus sp., Fusarium sp., Penicillium sp., Trichoderma sp., etc. Iron tolerance studies were carried out on Potato dextrose agar and Czapekdox agar using disc diffusion method with FeCl3 concentrations ranging from 200 to 1000 mg/l. Out of 14 isolates, 6 showed maximum tolerance at 1000 mg/l. The percentage of iron removal was maximum i.e. 96.62% by Aspergillus japonicus strain VIT-SB1 at 36 hrs at pH 6 and ambient temperature without any pre-treatment of fungal biomass. Hence, the isolated fungi exhibits great tolerance to iron and can be used successfully for bioremediation purpose.

Keywords

Aspergillus japonicus Biosorption Fungus Heavy metal contamination Iron Metal tolerance

Article Details

How to Cite
Panda, S. S. ., Basu, A. ., Patnaik , N. ., & Dhal , N. K. . (2017). Screening, biosorption and identification of indigenous fungal strains of iron mining area, Odisha, India. Environment Conservation Journal, 18(1&2), 211–218. https://doi.org/10.36953/ECJ.2017.181229

References

  1. Ahmad, I., Zafar, S., and Ahmed, F., 2005. Heavy metal biosorption potential of Aspergillus sp. and Rhizopus sp., isolated from wastewater treated soil. Journal of Applied Science and Environmental Management, 9(1):123-126.
  2. Al-Garni, S.M., Ghanem, K.M. and Bahobail, A.S., 2009. Biosorption characteristics of Aspergillus fumigatus in removal of cadmium from an aqueous solution. African Journal of Biotechnology, 8(17): 4163-4172
  3. Barea, J.M. and Jeffries, P., 1995. Arbuscular mycorrhizas in sustainable soil plant systems. In: Hock B, Varma A (eds) Mycorrhiza structure, function, molecular biology and biotechnology. Springer, Heidelberg, pp 521–559.
  4. Barnett, H.L. and Hunter, B.B., 1999. Illustrated genra of imperfect fungi.Fourth edition. Prentice Hall Inc.
  5. Basu, A., Panda, S.S. and Dhal, N.K., 2015. Effect and Accumulation of Lanthanum on the Growth and Physiological activities of Cymbopogon flexuosus (Nees ex Steud.)W.Watson. Current World Environment, 10(3): 951-956
  6. Caánovas, D., Durán, C., Rodríguez, N., Amils, R. and Lorenzo, V., 2003. Testing the limits of biological tolerance to arsenic in a fungus isolated from the River Tinto. Environmental Microbiology. 5(2):133-138.
  7. Chen, G., Ushida, T. and Tateishi, T., 2002. Scaffold design for tissue engineering. Macromolecular Bioscience, 2(2):67–77.
  8. Durán, C., Marín, I. and Amils, R. 1999. Specific metal sequestering acidophilic fungi. Biohydrometallurgy and the Environment Toward the Mining of the 21st Century. (Eds.): R. Amils and A. Ballester. Elsevier, Amsterdam. 521-530.
  9. Gadd, G.M., 1990. Fungi and yeasts for metal accumulation. In Microhiul Mineral Recovery, ed. H. L. Ehrlich & C. L. Brierley. McGraw-Hill, New York, pp. 249-76.
  10. Gadd, G.M., 1993. Interaction of fungi with toxic metals. New Phytology, 124: 25-60.
  11. Gerhardt, P., Murray, R.G.E., Wood, W.A. and Krieg, N.R., 1994. Methods for General and Molecular Bacteriology. ASM Press, Washington, D.C.
  12. Gupta, P.K., 2000. Chemical methods in environmental Analysis Water, Soil and Air. India, Agrobios. 240–241.
  13. Jackson, M.L., 1973. Soil chemical analysis. New Delhi, Prentice Hall Pvt. Ltd.
  14. Jiang, S., Wang, W., Xue, X., Cao, C., and Zhang, Y., 2015. Fungal diversity in major oil-shale mines in China. Journal of environmental sciences, 41:81-89
  15. Kapoor, A. and Viraraghavan, T., 1995. Fungal biosorption: An alternative treatment option for heavy metal bearing wastewater. Bioresource Technology, 53:195-206.
  16. Kapoor, A. and Viraraghavan, T., 1998. Biosorption of Heavy Metals on Aspergillus niger: Effect of Pretreatment. Bioresource Technology, 63(2): 109-113.
  17. Khan, A.G. and Khoo, C.S., 2000. Role of plants, mycorrhizae and phytochelators in heavy metal contaminated land remediation. Chemosynthesis, 41(1-2): 197-207.
  18. Khosravi, F., Savaghebi, G.H., and Farah, B.H., 2009. Effect of Potassium chloride on Cd uptake by colza in a polluted soil. Water Soil Journal, 23:28–35.
  19. Mamman, S., Abdullahi, I. and Ukwede, R.O., 2011. Isotherms and batch kinetics of the biosorption of cadmium onto pre-treated tridax procumbens. Der Pharma Chemica. 3(4):94-101.
  20. Mehta, S. and Nautiyal, C.S., 2001. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Current Microbiology, 43(1): 51-56.
  21. Mohsenzade, F., Chehregani, A., and Akbari, M. 2012. Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils. Iranian Journal of Environmental Health Science and Engineering, 9(1):26–34.
  22. Paknikar, K.M., Palnitkar, U.S. and Puranik, P.R. 1993. In: Biohydrometallurgical Technologies (Eds.): A.E. Torma, M.L. Apel and C.L. Brierely. The Minerals, Metals and Materials Society, TMS Publications, Wyoming, USA.II: 229-236.
  23. Panda, P., Sahoo, L., Panda, S.K., 2008. Abiotic stresses in crop plants. Chapter 10, Heavy metal and metalloids stress in plant: The genomic perspectives; p. 164-177
  24. Panda, S.S., Sahoo, K., Muduli, S.D., Sahoo, G., Ahemad, M.D.J., Nayak, B.B. and Dhal, N.K. 2014. Chromium tolerant indigenous fungal strains from industrial effluents of anugul district, Odisha, India. Biolife, 2(2):634-640.
  25. Pradhan, S., Singh, S., and Rai, L.C., 2007. Characterization of various functional groups present in the capsule of Microcystis and study of their role in biosorption of Fe, Ni and Cr. Bioresource Technology 98(3):595–601.
  26. Sunani, G., Panda, S.S., Pattanayak, B., Dhal, N.K., 2015. Isolation, Characterization and Heavy Metal Tolerance Capacity of Indigenous Fungi: A Case Study of Iron Mine Waste. Journal International Environmental Application and Science, 10(2): 233-238
  27. Tamás, M.J., Labarre, J., Toledano, M.B., and Wysocki, R. 2005. Mechanisms of toxic metal tolerance in yeast. In: Tamás MJ, Martinoia E (eds) Molecular biology of metal homeostasis and detoxification: from microbes to man. Springer, Heidelberg, pp 395–454
  28. Vadkertiova, R. and Slavikova, E., 2006. Metal tolerance of yeasts isolated from water. Journal of Basic Microbiology, 46(2):145–152.
  29. Walkey, A. and Black, I.A., 1934. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science,37, 29–38.
  30. Wysocki, R., and Tamás, M.J., 2010. How Saccharomyces cerevisiae copes with toxic metals and metalloids. FEMS Microbiology Review, 34(6):925–951
  31. Xiezhi, Y., Jieming, C., Ming, H.M., 2005. Earthworm-mycorrhiza interaction on Cd uptake and growth of ryegrass. Soil Biology and Biochemistry, 37(2):195–201.
  32. Yan, G. and Viraraghavan, T., 2000. Effect of pretreatment on the bioadsorption of heavy metals on Mucor rouxi. Water SA, 26(1): 119-123.
  33. Zafar, S.F., Aqil, and Ahmad, I., 2007. Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agricultural Soil. Bioresource Technology, 98(13): 2557- 2561
  34. Zar, J.H., 1999. Biostatistical Analysis. New Fersey. 663 p.