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Submitted
May 15, 2024
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November 3, 2024
Published
November 25, 2024
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Abstract

Isolating high-quality genomic DNA from Abelmoschus esculentus (okra) is very hard because it contains a lot of acidic polysaccharides (mucilage) and polyphenols, which can make DNA extraction harder. This study selected twenty different genotypes of okra, carefully harvested healthy juvenile leaves, and washed them with distilled water to minimize contamination. A novel, cost-effective, rapid, and simplified method for DNA extraction was standardized, addressing the specific challenges posed by okra's biochemical composition in comparison to the conventional methods. The modified protocol yielded 20–30% more DNA compared to conventional methods, resulting in superior quality and purity.  The DNA isolated using this method was sufficiently pure for approximately 275-300 PCR reactions, enabling accurate downstream applications such as genetic diversity analysis. This optimized protocol represents a significant advancement in genomic DNA isolation from okra, providing a reliable and efficient alternative to conventional approaches.

Keywords

Abelmoschus esculentus CTAB Genomic DNA Modified technique

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Copyright (c) 2024 Environment Conservation Journal

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How to Cite
Sarthi, Johar, V., Singh, N., Alaklabi, A., & Khan, S. (2024). Rapid and efficient isolation of genomic DNA from Okra (Abelmoschus esculentus L.): A streamlined alternative to conventional methods. Environment Conservation Journal, 25(4), 1172–1179. https://doi.org/10.36953/ECJ.27652839
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