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This study explored the soil as a rich source of microorganisms capable of creating novel antibacterial chemicals in an effort to address the growing problem of antibiotic resistance. The isolation process involved careful collection and laboratory cultivation of a variety of soil samples from garbage, gardens, and agricultural settings. The techniques used for characterization included biochemical examination of metabolic characteristics, spore staining, lactophenol cotton blue staining, and Gram-tag staining. Using the well diffusion technique, the antibiotic-producing capacities of the strains were evaluated. Notable antibacterial activity was found for four bacterial isolates (B2, B5, B6, and B9) and seven fungal strains (2F, 3F, 4F, 5F, 7F, 9F, 10F, and 11F). Precise soil sampling and complex microbe cultivation and characterization are major obstacles. The unique aspect of the work is how well antibiotic-producing bacteria were isolated and described from a variety of soil samples, underscoring the possibility of using natural habitats as sources of novel antimicrobial agents. The detected antibacterial activity emphasizes how crucial it is to carry out additional research to combat antibiotic resistance. This study provides opportunities for additional research into the unexplored potential of soil microorganisms for the development of novel antimicrobial agents.


Antibiotics antimicrobial activity inhibition zone well diffusion method

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How to Cite
Gupta, A., Pandey, S., & Upadhyay, H. (2024). Tiny defenders: Isolating antibiotic producers from soil samples. Environment Conservation Journal, 25(2), 402–408.


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