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Abstract
Cascabela thevatica leaf part was tested for antibacterial efficacy against Escherichia coli organism using the disc diffusion method. The crude alcoholic extract was found effective against Escherichia coli organism. The antibacterial activity is attributed to the presence of alkaloids, which was confirmed by gas chromatography end positive alkaloid test. The minimum inhibitory concentration (MIC) was determined for the crude extract by paper disc diffusion method. The result were compared with reference antibiotic tetracycline (one unit strength).
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References
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- Ansari M. M. 1995. Control of sheath blight of rice by plant extract. Indian Phytopath. 48 (3), 268-270.
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- Ghanaksha A. and Kaushik P. 1999a. Antibacterial effect of Rhynchostylis retusa BL. (Orchidaceae). Advances in Plant. Sciences 12 (11), 593- 598
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- Kaushik P. 1983. Ecological and Anatomical Marvels of the Himalayan Orchids. Today and Tomorrow's Printers and Publisher, New Delhi pp viii +123+pltes 71.
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- Kaushik P. 1988. Indigenous Medicinal Plants Including Microbes and Fungi. Today and Tomorrow's Printer's and Publisher, New Delhi, pp vii+ 243.
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- Kaushik, P. and Dhiman A. K. 2000. Medicinal Plants and Raw Drugs of India. Bishen Singh Mahinder Pal Singh, Dehradun. Pp xii + 623.
- Kaushik P. and Kishore N. 1991. Antibacterial potential of pholidota articulata Lindi. A study in vitro. J. Orchid Society ofIndia. 5 (1, 2), 93-96.
- Kaushik P. and Upadhyay Vishal. 1999. Antibacterial activity of garlic ( Allium-sativuni) plant extract. In: D.R. Khanna, Ashutosh Gautam and A Goutam (eds.), Sustainable Ecosystem and Environment. Action for sustainable. Efficacious Development and Awareness (ASEA). Pp 129-132.
- Kaushi P, Upadyay Vishal and Padma Singh 2000. Antibacterial potential of Bryophytes, The Botanica, pp 1 3 1 -135.
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- Stahl E. 1969. Thin Layer Chromatography. 2" ed. MRF Ashworth Springer Verlag. Heidelber. DOI: https://doi.org/10.1007/978-3-642-88488-7
References
Ajay K. and. Kaushik P. 1999. Antibacterial effect of Adantum Capillus eneris Linn. Indian Fern J. 16, 72-74
Ansari M. M. 1995. Control of sheath blight of rice by plant extract. Indian Phytopath. 48 (3), 268-270.
Bauer A. W.. Kirbey, W. M M, Sherries J. C. and Turck 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal ofClinical Pathology. 45, 493. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
Chakraborty A. and. Brantner. A, H, 1999, Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens. Journal ofEthnopharmacology. 68, 339 --344. DOI: https://doi.org/10.1016/S0378-8741(99)00119-1
Chatterjee A and Pakrashi S. C. 1995 The Treatise on Indian Medicinal Plants. V-4, Publicatiion and Information Directorate, C.S.I.R., New Delhi
Chopra R. N., Nayar S. L. and Chopra l. C. 1956. Glossary ofIndian Medicinal Plants. Council of scientific and Industrial Reasearch, New Delhi.
Dinda B. and Saha S. I 990. Chemical constituents of Plumbago Zeylanica aerial parts and Thevatica nerrifolia roots. JI. Indian Chem. 67, 88-89.
Ebi G. C. and Ofoefule S. L. 1997. Investigation into folkloric anti microbial activities of Landolphia Owrrience. Phytotherapy Research 11 (2), 149-- 151, DOI: https://doi.org/10.1002/(SICI)1099-1573(199703)11:2<149::AID-PTR43>3.0.CO;2-N
Gehlot D and Bohra J. A. 1998. Antimicrobial activity of various plant parts extract ofAerva persica. Advances in Plant Sciences 11 (1), 109-111.
Ghanaksha A. and Kaushik P. 1999a. Antibacterial effect of Rhynchostylis retusa BL. (Orchidaceae). Advances in Plant. Sciences 12 (11), 593- 598
Ghanaksha A. and Kaushik P. 1999b. Antibacterial effect of Aerides multiflora Roxb : a study in vitro. .J. Orchid Soc. India. 13 (1-2), 765-68.
Kaushik P. 1983. Ecological and Anatomical Marvels of the Himalayan Orchids. Today and Tomorrow's Printers and Publisher, New Delhi pp viii +123+pltes 71.
Kaushik P. 1985. Glimpses of Medical Botany in Atharvaveda ( Kand IV). The Vedic Path. 48, 64-67.
Kaushik P. 1988. Indigenous Medicinal Plants Including Microbes and Fungi. Today and Tomorrow's Printer's and Publisher, New Delhi, pp vii+ 243.
Kaushik P 2000. Introductory Microbiology. Emkay Publications Post Box No. 9410, B-19 East Krishna Nagar. Swami Dayanand Marg, Delhi. Pp viii +466.
Kaushik P and Kishore N. 1995. Antibacterial activity of Dendrobium amoenum wall Ex. Lindi - A study in vitro. J. Orchid Soc. India. 9 (102), 33:35.
Kaushik, P. and Dhiman A. K. 2000. Medicinal Plants and Raw Drugs of India. Bishen Singh Mahinder Pal Singh, Dehradun. Pp xii + 623.
Kaushik P. and Kishore N. 1991. Antibacterial potential of pholidota articulata Lindi. A study in vitro. J. Orchid Society ofIndia. 5 (1, 2), 93-96.
Kaushik P. and Upadhyay Vishal. 1999. Antibacterial activity of garlic ( Allium-sativuni) plant extract. In: D.R. Khanna, Ashutosh Gautam and A Goutam (eds.), Sustainable Ecosystem and Environment. Action for sustainable. Efficacious Development and Awareness (ASEA). Pp 129-132.
Kaushi P, Upadyay Vishal and Padma Singh 2000. Antibacterial potential of Bryophytes, The Botanica, pp 1 3 1 -135.
Okunade A. L., Hufford C. D, Clark A. M. and Lantz D. 1997. Antimicrobial properties of constituents of Piper aduncum. Phytotherapy Research. 11(2), 142- 144. DOI: https://doi.org/10.1002/(SICI)1099-1573(199703)11:2<142::AID-PTR61>3.0.CO;2-Y
Peres M. T., Moncha A. B. Cruz Pizzelati M. G. and Yunes R. A. 1997. Chemical composition and anti microbial activity of Croton urucurana Baillon (Euphorbiaceae). Journal ofEthanopharmacology. 56 (3), 223-226 DOI: https://doi.org/10.1016/S0378-8741(97)00039-1
Stahl E. 1969. Thin Layer Chromatography. 2" ed. MRF Ashworth Springer Verlag. Heidelber. DOI: https://doi.org/10.1007/978-3-642-88488-7