Main Article Content

Abstract

Histopathology is an important diseases diagnostic tool. Penetration into the host is the first step for a microbial agent to mutiply and invade the vital organs of its host. Like all animals fishes suffers from environmental, stress, which is followed by pathogenic attacks and parasitic afflictions causing Epizootic Ulcerative syndrome (EUS). Histological investigations of tissues of EUS infected fish Channa punctatus showed an eroded epidermis, necrosis of skeletal muscles and intense granulomatous inflammation, infiltration of mixed inflammatory cells in muscular as well as submuscular layers of the skin. Also a characteristic focal grannular granuloma in the gill lamellae was noticed. The electron microscopic observations, of the tissue cells of the fishe showed viruses like Parvovirus even at a red spot stage. Thus, it can be said that Viruses being crystalline in nature have cellular penetrating power & the EUS infection follows the phyllogenetic evolution in life in true sense i.e. RNA virus followed by Bacterial cell and then Fungus. Thus, the presence of EUS in Channa punctatus is confirmed histologically.

Keywords

Channa Epizootic Ulcerative syndrome Fungus Histology Parvovirus

Article Details

How to Cite
Laharia, R. ., & Patel, P. . (2020). Histological changes in skin and gill of fresh water EUS infected fish Channa punctatus. Environment Conservation Journal, 21(1&2), 75–84. https://doi.org/10.36953/ECJ.2020.211208

References

  1. Baldock, F.C., Blazer, V., Callinan, R.B., Hatai, K., Karunasagar, I. & Mohan, C.V., 2005. Outcomes of a short expert consultation on epizootic ulcerative syndrome (EUS): re-examination of causal factors, case definition and nomenclature. In P. Walker, R. Lester & M.G. Bondad-Reantaso, eds. Diseases in Asian aquaculture V. Manila, Fish Health Section, Asian Fisheries Society, pp. 555–585.
  2. Blazer, V. S.; Vogelbein ,W. K.; Densmore, C.; Zwerner, D E.; May, E. B., 1999. Aphanomyces as a cause of ulcerative skin lesions in menhaden from Chesapeake Bay. J.Aquat. Anim. Health, Vol.1, pp. 340-349.
  3. Cheville, N., 1983. Cell Pathology, 2nd ed. Ames, IA-Iowa State University Press.
  4. Choongo, K., Hang’ombe, B., Samui, K.L., Syachaba, M., Phiri, H. & Maguswi, C., 2009. Environmental and climatic factors associated with epizootic ulcerative syndrome (EUS) in fish from the Zambezi floodplains, the Republic of Zambia. Bulletin of Environmental Contamination and Toxicology, Vol. 83, pp. 474–478.
  5. Dykstra, M. J.; Levine, J. F.; Noga, E. J.; Hawkins, J. H.; Gerdes, P.; Hargis, W.J Jr.; Grier, W. H. and Te Strake, D., 1989. Ulcerative mycosis : A serious Menhaden disease of the South-Eastern coastal fisheries of the United States. Journal of Fish Diseases, Vol. 2, pp. 175-178.
  6. Hasan M.A., M. Mamnur Rashid*, M.A. Islam, K. Mostafa and M. Tarikul Islam., 2008. Histopathological studies of EUS affected shing, Heteropneustes fossilis (Bloch) from a fish farm of Mymensingh, Bangladesh Bangladesh J. Fish. Res., Vol.12, No.1, pp. 21-26.
  7. Kanchanakhan, S., 1996. Epizootic Ulcerative Syndrome (EUS) : a new look at the old story . AAHRI Bangkok Newsletter; Vol. 5, No.1, pp. 2.
  8. Kanchanakhan, S.; Saduakdee, U. and Areerat, S., 1998. Virus Isolation from Epizootic Ulcerative Syndrome-Diseased Fishes. The AAHRI Newsletter Abstracts; December, 1998., Vol.7 , No.2.
  9. Kane, A. S.; Dykstra, M. J.; Noga. E. J.; Reimschuessel, R.; Baya, A.; Driscoll, C.; Paerl, H. W. and Landsberg, J., 2000. Etiologies, observations and reporting of estuarine finfish lesions. Marine Environmental Research, Vol. 50, pp. 473-477.
  10. Lio-Po, G. D.; Albright, L. J.; Michael, C. and Leano, E. M., 1998. Experimental induction of lesions in snakeheads (Ophicephalus striatus) and catfish, Clarias batrachus with Aeromonas hydrophila. Aquaspirillum sp., Pseudomonas sp. and streptococuss sp. J. Appl. Ichthyol., Vol.14, No.1 and 2, pp. 75-79.
  11. Roberts, R. J.; Campbell, B. and Macrae, J. H., 1994. Proceeding of the regional seminar on epizootic ulcerative syndrome. The Aquatic Animal Health Research Institute. Bangkok, January, pp. 25 – 27.
  12. Sammut, J.; White, I. and Melville, M. D.,1996. Acidification of an estuarine tributary in eastern Australia due to drainage of acid sulfate soils. Marine and Freshwater Research; Vol. 47, No 5, pp. 669 – 684.
  13. Songe, M.M., Hang’ombe, M.B., Phiri, H., Mwase, M., Choongo, K., Van der Waal, B., Kanchanakhan, S., Reantaso, M.B. & Subasinghe R.P., 2012. Field observations of fish species susceptible to epizootic ulcerative syndrome in the Zambezi River basin in Sesheke District of Zambia. Tropical Animal Health & Production, Vol. 44, pp. 179–183. DOI 10.1007/s11250-011- 9906-1.
  14. Viswanath, T. S.; Mohan, C. V. and Shankar, K. M., 1997. Clinical and histopathological characterization of different types of lesions associated with Epizootic Ulcerative Syndrome (EUS). Journal of Aquaculture in the Tropics.; Vol. 12, No. 1, pp. 35 – 42.
  15. Vogelbein, W. K.; Shields, J. D.; Has, L. W.; Reece, K. S. and Zwerner, D. E., 2001. Skin Ulcers in Estuarine Fishes : A Comparative Pathological Evaluation of Wild and laboratory-Exposed Fish. Environmental Health Perspectives. Vol. 109:,suplement 5, pp. 687-693.
  16. Weissman, K., 1972. General zoological microtechniques. Pub., Blkiston Company, Philadelphia, pp. 22.