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Abstract
The In vitro antibacterial activity of Cascabela thevatica plant extract has been investigated against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae using the agar disc diffusion method. The alcoholic extract was found effective against Escherichia coli, Staphylococcus aureus and Salmonella typhi but does't show any activity against lebsiella pneumoniae. The antibacterial activity is attributed to the presence of alkaloids, which was confirmed by gas liquid chromatography (GLC) and positive alkaloid test. The minimum inhibitory concentration (MIC) was determined by paper disc diffusion method and the results were compared with reference antibiotic tetracycline (one unit solution).
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References
- Bauer,A. W.,Kirby.W.M.M, Sherris, J.C.and Turck 1966: Antibiotic susceptibility testing by standardized single disc method. American Journal of clinical pathology. 45: 493. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
- Chakraborty.A.,andBrantner, A.H,, 1999: Journal ofEthnopharmacology 68 : 339-344. Dhar, M.N., Dhar M.M., Dhawan, B.N., Mahrotra, B.N., and Ray, C, 1968: Screening of Indian plants for biological activity. Part-1. Indian J.Expt. Biology 6, 232.
- Gahlot, D., and Bohra,A 1998: Advances in PlantSciences. 11(1): 109-111. Kaushik, P, and Kishor, N., 1991: J. Orchid Society ofIndia, 5 (1,2): 93-96. Machat, D.I., and Kankel, W.H., 1920: Proc. Sec. Exp. Bio. Med. 19, 68 .
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References
Bauer,A. W.,Kirby.W.M.M, Sherris, J.C.and Turck 1966: Antibiotic susceptibility testing by standardized single disc method. American Journal of clinical pathology. 45: 493. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
Chakraborty.A.,andBrantner, A.H,, 1999: Journal ofEthnopharmacology 68 : 339-344. Dhar, M.N., Dhar M.M., Dhawan, B.N., Mahrotra, B.N., and Ray, C, 1968: Screening of Indian plants for biological activity. Part-1. Indian J.Expt. Biology 6, 232.
Gahlot, D., and Bohra,A 1998: Advances in PlantSciences. 11(1): 109-111. Kaushik, P, and Kishor, N., 1991: J. Orchid Society ofIndia, 5 (1,2): 93-96. Machat, D.I., and Kankel, W.H., 1920: Proc. Sec. Exp. Bio. Med. 19, 68 .
Singh, P., and Karnwal, P., 2006: Antifungal activity of Cassia.fistula leaf extract against Candida albicans, Indian Journal ofMicrobiology. Vol 46, No 2, June 06, pp 169-170. Stahl E.,1969: 2"" ed. MRF Ashworth Springer Verlag, Heidelber.