Histochemical demonstration of protein concentration in intestinal tissue of coccidiosis infected broiler chicks treated with Mercurius corrosivus


S. T. Naphade


The present investigation includes the study of protein concentration in intestinal tissue of coccidiosis infected broiler chicks orally inoculated by the infection of Eimeria tenella sporulated oocysts and treated with Mercurius corrosivus by using the methods of histochemical techniques. The treatment of homoeopathic medicine Mercurius corrosivus was administered to the coccidiosis infected group of broiler chicks.For the study of protein concentration in intestinal tissue of the coccidiosis infected and non-infected broiler chicks, the histochemical technique was used. Histochemical study indicates that the presence of different concentration of protein content found in the intestinal tissue of the broiler chicks.Intestinal tissue of all the chicks of different groups have presence of protein content in variable concentrations. It is also observed that the presence of protein concentration was variable in different region and shows different traces like minimum, medium and maximum amount of protein content found in different region of the intestinal tissue.The amount of protein concentration was observed high in group B(INC) in comparison to the other group of chicks observed during the experimental period. The concluded results of the study by using histochemical techniques shows that the protein concentration in the intestinal tissue is demonstrated by the variable staining reaction. The concentration of protein content is maximum in the parasitic stages showing the utilization of the protein from the host tissue. Whereas the infected and treated group with homoeopathic medicine Mercurius corrosivus also showed variable staining reaction according to the presence of protein in the intestinal tissue.


How to Cite
Naphade, S. T. . (2020). Histochemical demonstration of protein concentration in intestinal tissue of coccidiosis infected broiler chicks treated with Mercurius corrosivus. Environment Conservation Journal, 21(3), 113-117. https://doi.org/10.36953/ECJ.2020.21313


  1. Al-Saffar, F. J., Eyhab, R. M. and Al-Samawy, 2016. Histomorphological and histochemical study of the small intestine of the striated scope owls (OtusScorsBrucei). Singapore Journal of Chemical Biology, 5: 1-10.
  2. Bonhag, P. F. 1955. Histochemical studies of the ovarian nurse tissue and oocyte of the milkweed bug, Oncopeltus fasciatus (Dallas). Journal of Morphology, 96 (3): 381-439.
  3. Chaudhuri, N. M. 1990. A Study on Materia Medica, Publisher: B. Jain Publishers Pvt. Ltd., New Delhi.
  4. Day Christhoper, 1988. The homeopathic treatment of small animals principles and practice. B. Jain Publishers (Pvt.) Ltd. New Delhi.
  5. Farrington, E. A. 1989. Clinical Materia Medica, XIV Edition Revised and enlarged by Harrey Farrington, B. Jain Publishers Pvt. Ltd., New Delhi, 55.
  6. Gill, B. S. and Bajwa, R. S. 1979. Drug resistance in field isolates of chicken coccidia from Punjab State. Indian Journal of Parasitology, 3: 131-134.
  7. Hodges, R. D. 1974. The Histology of the Fowl, Academic Press, Edition.
  8. Madrewar, B. P. 1996. Therapeutics of Veterinary Homoeopathy, B. Jain Publishers (P) Ltd. New Delhi.
  9. Mukharji, K. L. 1990. Medical Laboratory Technology Edition IP 1138 Tata Mcgrawhill publishing Co. Ltd. New Delhi.
  10. Naveen, P. K. 2005. The relevance of homoeopathy in veterinary therapeutics and safe animal food production, proceedings of National seminar on Application of homoeopathy in birds, fishes, plants, soil, and environment held at Thrissur, Kerla.
  11. Narahari, D. 1996. Commercial broiler production. Emkay Publications, Delhi.
  12. Pellerdy, L. P., 1974. Coccidia and coccidiosis, Verlagpaulparey, No 2nd edition, pp. 959.
  13. Singh, J., Bajwa, R. S. and Gill, B. S. 1982. Effect of druges against coccidial infection of chicken and the resultant immunity. Indian Journal of Parasitology, 6: 183-190.
  14. Singh, U. B, and Sulochana, S. C. 1997. Handbook of Histological and Histochemical techniques Edition II, pp. 39-41. Premier Publishing House, S-l-800, 1st floor, Kothi, Hyderabad-95.
  15. Williams, R. B., Carlyle, W. W., Bond, W. W., Brown; D. R. 1999. The efficacy and economic benefits of paracox, a live attenuated anticoccidial vaccine, in commercial trials with standard broiler chickens in the United Kingdom. International Journal for parasitology oxford, 29: 341-355.