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Abstract
The in vitro antibacterial activity of Rauwolfia serpentina plant extract has been investigated against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae using the disc diffusion method. The alcoholic extract was found effective against Staphylococcus aureus only. The antibacterial activity is attributed to the presence of alkaloids, which was confirmed by gas liquid chromatography and positive alkaloid test. The minimum inhibitory concentration (MIC) was determined by paper disc diffusion method. The results were compared with reference antibiotic tetracycline (one unit solution).
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References
- Ansari, M.M., 1995. Control of sheath bright of rice by plant extract. Indianphytopathology, 48(3): 268•270.
- Bauer, A.W., Kirby, W.M.M., Sherris J.C. and Turek, 1966.Antibiotic susceptibility testing by standardized single disc method. American Journal ofClinical Pathology,45: 493. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
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References
Ansari, M.M., 1995. Control of sheath bright of rice by plant extract. Indianphytopathology, 48(3): 268•270.
Bauer, A.W., Kirby, W.M.M., Sherris J.C. and Turek, 1966.Antibiotic susceptibility testing by standardized single disc method. American Journal ofClinical Pathology,45: 493. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
Chakraborty, A. and Brantner, A.H., 1999. Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens. Journal of Ethnaopharmacology, 68: 339•344. DOI: https://doi.org/10.1016/S0378-8741(99)00119-1
Gehlot, D. and Bohra, A., 1998. Antimicrobial activity of various plant part extracts of Aerva persica. Advances in plant science, I J (I): 109-1 11
Kaushik, P. and Dhiman, A.K., 1999. Medicinal plants and raw drugs of India.Bishan Singh, Mahinder Pal Singh, Dehradun, xii+623.
Machat, D.I and Kuundal, W.M., 1920. Proc.Soc.Exp.Bio Med., 19-68.
Stahi, E., 1969 Thin layer chromatography 2 edition: MRF Ashworth Springer Verlag., Heidelber.