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Abstract
In the present study authors studied the effect of bio-pesticide (Agroneem) on the biochemistry of kidney of Clarias batrachus. The biochemical parameters studied were RNA, total protein, acid phosphatase, alkaline phosphatase, GOT and GPT. These parameters in the experimental animal were found decline. The order of toxicity of Agroneem to biochemical parameters from higher to lower were- acid phosphatase (-78.623%)< total protein (-78.078%)< alkaline phosphatase (-70.594%)< RNA (-44.049%)< GPT (-39.618%)< GOT (-29.948%). Thus on basis of obtained results in the present investigation it can be concluded that 96 hrs. exposure of 30 ppm of Agroneem aqueous solution has toxic effect and alter the biochemistry of kidney. Therefore, it is recommended to the user of this bio-pesticide that they should be careful about the dose they are using.
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References
- Attri, B.S. and Ravi Prasad, G, 1980. Neem oil extractive an effective mosquito larvicide. Indian J. Ent., 42(3): 371•374.
- Deshmukh, P.B. and Pariyal, K.G, 1992. Acute toxicity of Neemark to a freshwater fish. Tlapia mossambica. Pestology, 16(7): 28-30.
- Farah, M.A. and Ateeq, B. and Ahmad W, 2006. Antimutagenic effect of neem leaves extract in freshwater fish, Channa punctatus evaluated by cytogenetic tests. Sci. Total Environ.,364(1-3): 200-214. DOI: https://doi.org/10.1016/j.scitotenv.2005.07.008
- Gandhi, M., Lal, R, Sankarnarayanan, A, Banerjee, C.K. and Sharma, PL., 1988. Acute toxicity study of the oil from Azadirachta indica seed (neem oil). J. Ethropharmacology.,23: 39-51 DOI: https://doi.org/10.1016/0378-8741(88)90113-4
- Jayaraman, J,, 2000. Laboratory manual in biochemistry. New Age International Publication. pp: 1-180.
- Kanungo, D., 1996. Pharmacology and Toxicology. In Neem Eds. N.S. Pradhan and B.S. Parmar. pp: 293-309
- Kasutri, M., Ahmed, R.N., Pathan, K.M., Shaik, P.D. and Manivannan, B., 1997. Effect of Azadirachta indica leaves on the seminal vesicles and ventral prostate in albino rates. India J. Physiol. Pharmacol., 41(3): 234-240.
- Miller, F. and Vetz, S., 1998. Evaluating Biorational Pesticides for controlling Arthropod Pest and their Phytotoxic Effects on Green house crops. Hort. Technology., 8(2): 185-192. DOI: https://doi.org/10.21273/HORTTECH.8.2.185
- Rahman, M.F, Siddiqui, M.K. and Jamil, K., 200L. Effects of Vepacide (Azadirachta indica) on aspartate and alanine aminotransfer are profiles in a subchronic study with rats. Hum. Exp. Toxicol, 20(5): 243-249. DOI: https://doi.org/10.1191/096032701678227730
- Wan, M.T., Watls, R.G, Isman, M.B. and Strub, R., 1996. Evaluation of the acute toxicity to Juvenile North West Salmon of Azadirachtin. Neem Extract, and Neem Based Products. Bull. Environ. Contain Toxicol, 56: 432-439. DOI: https://doi.org/10.1007/s001289900062
References
Attri, B.S. and Ravi Prasad, G, 1980. Neem oil extractive an effective mosquito larvicide. Indian J. Ent., 42(3): 371•374.
Deshmukh, P.B. and Pariyal, K.G, 1992. Acute toxicity of Neemark to a freshwater fish. Tlapia mossambica. Pestology, 16(7): 28-30.
Farah, M.A. and Ateeq, B. and Ahmad W, 2006. Antimutagenic effect of neem leaves extract in freshwater fish, Channa punctatus evaluated by cytogenetic tests. Sci. Total Environ.,364(1-3): 200-214. DOI: https://doi.org/10.1016/j.scitotenv.2005.07.008
Gandhi, M., Lal, R, Sankarnarayanan, A, Banerjee, C.K. and Sharma, PL., 1988. Acute toxicity study of the oil from Azadirachta indica seed (neem oil). J. Ethropharmacology.,23: 39-51 DOI: https://doi.org/10.1016/0378-8741(88)90113-4
Jayaraman, J,, 2000. Laboratory manual in biochemistry. New Age International Publication. pp: 1-180.
Kanungo, D., 1996. Pharmacology and Toxicology. In Neem Eds. N.S. Pradhan and B.S. Parmar. pp: 293-309
Kasutri, M., Ahmed, R.N., Pathan, K.M., Shaik, P.D. and Manivannan, B., 1997. Effect of Azadirachta indica leaves on the seminal vesicles and ventral prostate in albino rates. India J. Physiol. Pharmacol., 41(3): 234-240.
Miller, F. and Vetz, S., 1998. Evaluating Biorational Pesticides for controlling Arthropod Pest and their Phytotoxic Effects on Green house crops. Hort. Technology., 8(2): 185-192. DOI: https://doi.org/10.21273/HORTTECH.8.2.185
Rahman, M.F, Siddiqui, M.K. and Jamil, K., 200L. Effects of Vepacide (Azadirachta indica) on aspartate and alanine aminotransfer are profiles in a subchronic study with rats. Hum. Exp. Toxicol, 20(5): 243-249. DOI: https://doi.org/10.1191/096032701678227730
Wan, M.T., Watls, R.G, Isman, M.B. and Strub, R., 1996. Evaluation of the acute toxicity to Juvenile North West Salmon of Azadirachtin. Neem Extract, and Neem Based Products. Bull. Environ. Contain Toxicol, 56: 432-439. DOI: https://doi.org/10.1007/s001289900062