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December 10, 2008
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June 16, 2009
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Abstract

In  the  present study,  gram-negative  (Escherichia coli K-12)  bacterial biomembrane  involvement was studied  in  the presence of modulating  factors such as EDTA, Mg   ions  and EDTA and Mg'  ions in  combination.  The  release  of proteins  and  their  involvement during the  transport of 9-Lactams namely  Ceftriaxone  and  Cefazolin  were  also  studied.  The  broader  applications   of Ceftriaxone  for pharmaceutical  implications  offer  greater  advantage as  compared  to pre-existing  3-Lactams.  Due to the  availability  of more signal  moi.:cules  in the membranes  there  is  enhanced  toxicity  at 5  mM EDTA  concentration,  and  easy  entrapment of antibiotics, thus  enhanced sensitivity  levels.  A concentration  of 15  mM Mg  ions  was found  to be toxic  for E.coli  whereas  it exhibited  luxuriant growth with decreasing  Mg'  ion  concentration  under antibiotic  stress. On the contrary, when 5  mM      • EDTA is  treated  in combination  wnth  Mg,  it  attributed  reduced  signals  available  on the membrane surface  therefore,  reduced  drug  sensitivity.  To identify  the  involvement  of specific  proteins  and  to know the site of proteins  released which are directly or indirectly involved in transport  of antibiotics across  the  biological  membrane,  the  protein  release  was  monitored  from  intact  cells,  as well  as, membrane vesicles derived from E.colt cells and studied upto a level of molecular weight determination and  measured   by using  a high-pressure  liquid  chromatography  (HPLC).  The  study confirms  the induction of certain  stress  signal  proteins  from the outer  membrane,  thereby  rendering the bacteria more  susceptible to therapy.

Keywords

High pressure liquid chromatography CEF- Ceftriaxone Sodium CEZ-Cefazolin Sodium Outer Membrane Proteins- OMPs

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Arora, B. (2009). Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain. Environment Conservation Journal, 10(1&2), 1–8. https://doi.org/10.36953/ECJ.2009.101201
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