Article Sidebar
Main Article Content
Abstract
14 plant and bacterial species were extensively investigated for α-amylase enzyme present in them. Comparison was made on the basis of optimum pH, optimum temperature, Km, Vmax, pH stability range and thermal stability of enzyme. It was found that the plant enzyme is equally good than the bacterial enzyme in terms of characteristics.
Keywords
Article Details
Copyright (c) 2010 Environment Conservation Journal
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
- Abe, J.I., Onitsuka, N., Nakano, T., Shibata, Y., Hizukuri, S., and Entani, E. 1994 Purification and Characterisation of Periplasmic α-amylase from Xanthomonas campestris K-11151” Journal of Bacteriology 176(12): 3584-3588 DOI: https://doi.org/10.1128/jb.176.12.3584-3588.1994
- Anto, H., Trivedi U. and Patel, K. 2006. Alpha- amylase production by Bacillus cereus MTCC 1305 using Solid State Fermentation. Food Technology Biotechnology 44(2): 241-245
- Biazus, JPM., Souza, RR, Marques, JE, Franco, TT, Santana, JCC., Tambourgi, E. B.2009. Production and characterization of amylases from Zea mays malt. Brazilian Archives of Biology and Technology 52(4): DOI: https://doi.org/10.1590/S1516-89132009000400024
- Femi-Ola, T.O and Olowe B.M., 2011. Characterisation of alpha-amylase from Bacillus subtilis BS5 isolated from Amitermes evvuncifer silvestri. Research Journal of Microbiology 6(2):140-146 DOI: https://doi.org/10.3923/jm.2011.140.146
- Freer, S.N., 1993. Purification and characterization of the extracellular alpha-amylase from Streptococcus bovis JB1. Applied Environmental Microbiology 59(5): 1398–1402. DOI: https://doi.org/10.1128/aem.59.5.1398-1402.1993
- Gangadharan, D., Nampoothiri, K.M., Sivaramakrishnan, S., and Pandey, A 2009. Biochemical Characterization of Raw-starch-digesting Alpha Amylase Purified from Bacillus amyloliquefaciens. Applied Biochemistry and Biotechnology 158: 653-662. DOI: https://doi.org/10.1007/s12010-008-8347-4
- Glymph, J. L. and Stutzenberger, F. J. 1977. Production, Purification and Characterisation of alpha-amylase from Thermomonospora curvata. Applied and Environmental Microbiology 34(4): 391-397 DOI: https://doi.org/10.1128/aem.34.4.391-397.1977
- Goyal, M. and Dobriyal, A.K. 2010. Partial Purification and Characterisation of low molecular weight α-amylase from Dolichos biflorus. Journal of Applied and Natural Sciences (In press)
- Kumar, A., Singh, V.K., Fitter, J., Polen, T., and Kayastha, A.M., 2010. α-amylase from germinating soybean (Glycine max) seeds-Purification, characterization and sequential similarity of conserved and catalytic amino acid residues. Phytochemistry 71(14-15):1657-1666. DOI: https://doi.org/10.1016/j.phytochem.2010.06.012
- Mohammed, S.A., Al-Malki, A.L and Kumosani, T.A. 2009. Partial Purification and characterization of five α-amylases from a wheat local variety (Balady) During germination. Australian Journal of Basic and Applied Sciences 3(3): 1740-1748.
- Nirmala, M and Muralikrishna, G. 2003. Three alpha-amylases from malted finger millet (Ragi, Eleusine coracana, Indaf-15)-purification and partial characterization. Phytochemistry 62 (1): 21-30 DOI: https://doi.org/10.1016/S0031-9422(02)00443-0
- Nour, M.E.M.E.I. and Yaqub, S.O. 2010. Partial Purification and Characterisation of α and β-Amylases isolated from Sorghum bicolor cv (Feterita) Malt. Journal of Applied Sciences 10(13):1314-1319 DOI: https://doi.org/10.3923/jas.2010.1314.1319
- Welker, N.E. and Campbell L. Leon 1967. Comparison of alpha-amylase of Bacillus subtilis and Bacillus amyloliquefaciens. Journal of Bacteriology 94(4): 1131-1135. DOI: https://doi.org/10.1128/jb.94.4.1131-1135.1967
References
Abe, J.I., Onitsuka, N., Nakano, T., Shibata, Y., Hizukuri, S., and Entani, E. 1994 Purification and Characterisation of Periplasmic α-amylase from Xanthomonas campestris K-11151” Journal of Bacteriology 176(12): 3584-3588 DOI: https://doi.org/10.1128/jb.176.12.3584-3588.1994
Anto, H., Trivedi U. and Patel, K. 2006. Alpha- amylase production by Bacillus cereus MTCC 1305 using Solid State Fermentation. Food Technology Biotechnology 44(2): 241-245
Biazus, JPM., Souza, RR, Marques, JE, Franco, TT, Santana, JCC., Tambourgi, E. B.2009. Production and characterization of amylases from Zea mays malt. Brazilian Archives of Biology and Technology 52(4): DOI: https://doi.org/10.1590/S1516-89132009000400024
Femi-Ola, T.O and Olowe B.M., 2011. Characterisation of alpha-amylase from Bacillus subtilis BS5 isolated from Amitermes evvuncifer silvestri. Research Journal of Microbiology 6(2):140-146 DOI: https://doi.org/10.3923/jm.2011.140.146
Freer, S.N., 1993. Purification and characterization of the extracellular alpha-amylase from Streptococcus bovis JB1. Applied Environmental Microbiology 59(5): 1398–1402. DOI: https://doi.org/10.1128/aem.59.5.1398-1402.1993
Gangadharan, D., Nampoothiri, K.M., Sivaramakrishnan, S., and Pandey, A 2009. Biochemical Characterization of Raw-starch-digesting Alpha Amylase Purified from Bacillus amyloliquefaciens. Applied Biochemistry and Biotechnology 158: 653-662. DOI: https://doi.org/10.1007/s12010-008-8347-4
Glymph, J. L. and Stutzenberger, F. J. 1977. Production, Purification and Characterisation of alpha-amylase from Thermomonospora curvata. Applied and Environmental Microbiology 34(4): 391-397 DOI: https://doi.org/10.1128/aem.34.4.391-397.1977
Goyal, M. and Dobriyal, A.K. 2010. Partial Purification and Characterisation of low molecular weight α-amylase from Dolichos biflorus. Journal of Applied and Natural Sciences (In press)
Kumar, A., Singh, V.K., Fitter, J., Polen, T., and Kayastha, A.M., 2010. α-amylase from germinating soybean (Glycine max) seeds-Purification, characterization and sequential similarity of conserved and catalytic amino acid residues. Phytochemistry 71(14-15):1657-1666. DOI: https://doi.org/10.1016/j.phytochem.2010.06.012
Mohammed, S.A., Al-Malki, A.L and Kumosani, T.A. 2009. Partial Purification and characterization of five α-amylases from a wheat local variety (Balady) During germination. Australian Journal of Basic and Applied Sciences 3(3): 1740-1748.
Nirmala, M and Muralikrishna, G. 2003. Three alpha-amylases from malted finger millet (Ragi, Eleusine coracana, Indaf-15)-purification and partial characterization. Phytochemistry 62 (1): 21-30 DOI: https://doi.org/10.1016/S0031-9422(02)00443-0
Nour, M.E.M.E.I. and Yaqub, S.O. 2010. Partial Purification and Characterisation of α and β-Amylases isolated from Sorghum bicolor cv (Feterita) Malt. Journal of Applied Sciences 10(13):1314-1319 DOI: https://doi.org/10.3923/jas.2010.1314.1319
Welker, N.E. and Campbell L. Leon 1967. Comparison of alpha-amylase of Bacillus subtilis and Bacillus amyloliquefaciens. Journal of Bacteriology 94(4): 1131-1135. DOI: https://doi.org/10.1128/jb.94.4.1131-1135.1967