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Abstract
Ashwagandha [Withania somnifera (L.) Dunal] is an important medicinal plant and its medicinal properties have been attributed to various classes of withanolide compounds. In the present study, we evaluated antifungal and antibacterial activities of withanolide extracted from roots of ashwagandha, against five pathogenic, fungi and bacteria. The minimal inhibitory concentration (MIC) of withanolides against two fungi, F. oxysporum and A. brassica were observed to be 4826.25 and 4474.22 ppm respectively. Antibacterial activity of withanolide extract was tested against three bacteria, E. coli¸ Pseudomonas solanacearum and Pseudomonas vulgaris. The maximum zone of inhibition at 4000 ppm of withanolide extract against E. coli, P. solanacearum and P.vulgaris were observed to be 8.7mm, 12.1mm and 12.5mm respectively. Thus withanolides extract was found to be the inhibitor of pathogenic fungi and bacteria.
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References
- Arora, S., Dhillon, S., Rani,G. and Nagpal, A.,2004. The in vitro antibacterial/synergistic activiyies of Withania somnifera extracts. Fitoterapia, 75:385-388. DOI: https://doi.org/10.1016/j.fitote.2004.01.002
- Atta-ur-rahman and Choudhary, M., 1993. New withanolides from Withania somnifera spp. J. of Natural products., 56: 1000-1006 DOI: https://doi.org/10.1021/np50097a003
- Choudhary, M.I., Dur-e-Shahwar, Parveen, Z. and Atta-ur Rahman., 1995. Antifungal steroidal lactones from Withania coagulans. Phytochemistry, 40 (4): 1243-1246. DOI: https://doi.org/10.1016/0031-9422(95)00429-B
- Dash, V.B. and Junins, A.M.M., 1983. A handbook of Ayurveda, Concept Publishing, New Delhi, 15-25.
- Finhold, R.W., 1951. Improved toximetric agar disk test for evaluation of wood preservative. Anal. Chem., 23: 1038. DOI: https://doi.org/10.1021/ac60055a037
- Gupta, A.K., Verma, R.K., Mishra, H.O. and Gupta, M.M. ,1996. Quantitative determination of Withaferin-A indifferent plant parts of Withania somnifera by TLC densitometry. JMAPS., 18: 788-790.
- Kazutoshi, Shibata, B. and Kaorn Umehara B., 1999. Cell differentiation inducing steroids from Withania somnifera. Chem. Parm. Bull., 47(11): 1646-1649. DOI: https://doi.org/10.1248/cpb.47.1646
- Ramteke, A.P.,Kalore, B.R. and Patil,S.R., 2003. Evaluation of antifungal activity of W.somnifera root extract against the fungal root infection in citrus. JNKVV-Reasearch Journal, 37(1):109-110.
- Ray, A.B. and Gupta, M., 1994. Structural features and classification of withanolides from Withania somnifera Dun. Prog. Chem. Org. Nat. Prod., 63: 1-3.
- Singh, S. and Kumar, S., 1998. Withania somnifera: The Indian ginseng Ashwagandha, Central Institute of Medicinal and Aromatic plants, Lucknow, India..
- Thornberry, H.H., 1950. A paper-disk plate method for quantitative evaluation of fungicide, and bactericides. Phytopathology, 40: 419.
References
Arora, S., Dhillon, S., Rani,G. and Nagpal, A.,2004. The in vitro antibacterial/synergistic activiyies of Withania somnifera extracts. Fitoterapia, 75:385-388. DOI: https://doi.org/10.1016/j.fitote.2004.01.002
Atta-ur-rahman and Choudhary, M., 1993. New withanolides from Withania somnifera spp. J. of Natural products., 56: 1000-1006 DOI: https://doi.org/10.1021/np50097a003
Choudhary, M.I., Dur-e-Shahwar, Parveen, Z. and Atta-ur Rahman., 1995. Antifungal steroidal lactones from Withania coagulans. Phytochemistry, 40 (4): 1243-1246. DOI: https://doi.org/10.1016/0031-9422(95)00429-B
Dash, V.B. and Junins, A.M.M., 1983. A handbook of Ayurveda, Concept Publishing, New Delhi, 15-25.
Finhold, R.W., 1951. Improved toximetric agar disk test for evaluation of wood preservative. Anal. Chem., 23: 1038. DOI: https://doi.org/10.1021/ac60055a037
Gupta, A.K., Verma, R.K., Mishra, H.O. and Gupta, M.M. ,1996. Quantitative determination of Withaferin-A indifferent plant parts of Withania somnifera by TLC densitometry. JMAPS., 18: 788-790.
Kazutoshi, Shibata, B. and Kaorn Umehara B., 1999. Cell differentiation inducing steroids from Withania somnifera. Chem. Parm. Bull., 47(11): 1646-1649. DOI: https://doi.org/10.1248/cpb.47.1646
Ramteke, A.P.,Kalore, B.R. and Patil,S.R., 2003. Evaluation of antifungal activity of W.somnifera root extract against the fungal root infection in citrus. JNKVV-Reasearch Journal, 37(1):109-110.
Ray, A.B. and Gupta, M., 1994. Structural features and classification of withanolides from Withania somnifera Dun. Prog. Chem. Org. Nat. Prod., 63: 1-3.
Singh, S. and Kumar, S., 1998. Withania somnifera: The Indian ginseng Ashwagandha, Central Institute of Medicinal and Aromatic plants, Lucknow, India..
Thornberry, H.H., 1950. A paper-disk plate method for quantitative evaluation of fungicide, and bactericides. Phytopathology, 40: 419.