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July 12, 2011
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December 22, 2011
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Abstract

In the present investigation xylanase producing bacteria was isolated from compost. A total of 95 xylanolytic bacteria were isolated on oat spelt xylan agar medium and screened by the xylanolysis method. Out of these 95 isolates, only one bacterial isolates, strain C1 was selected for further study on the basis of zone of hydrolysis on xylan-congo red agar plate. This strain was identified by 16S rDNA analysis. The phylogenetic analysis using 16S rDNA sequence data showed that isolate C1 showed highest nucleotide identity of 98% with Bacillus licheniformis strain CICC 10181 (GenBank accession no. GQ375235) and identified as Bacillus licheniformis strain C1. Bacillus licheniformis strain C1 was gram positive and rod shaped. Morphology of Bacillus licheniformis strain C1 showed- smooth texture, medium size, opaque transparency, creamish-white colour and serrated margin. Maximal xylanase production for Bacillus licheniformis strain C1 was achieved at the incubation period of 48 h. Xylanase and cellulase activities were determined as 20.0 U/ml and 1.3 U/ml, respectively.  The optimum pH and optimum temperature for xylanase activity was found to be 7.0 and 60°C, respectively. Xylanase was found to be thermostable at 60°C for 1h and retained 90% of its activity upto 6 h at this temperature. Approximately, 74% and 70% of its activity was retained at 70°C and 80°C respectively, after 6 h of incubation. All of these properties of the Bacillus licheniformis strain C1 xylanase make the suitability of this enzyme for its use in feed and baking industry.

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Feed and baking industry phylogenetic analysis 16S rDNA analysis xylanase

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Singh, V., Srivastava, K., Verma, A., & Agarwal, S. (2011). Identification of xylanase producing Bacillus licheniformis strain C1 and properties of crude xylanase. Environment Conservation Journal, 12(3), 113–120. https://doi.org/10.36953/ECJ.2011.120321
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