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Abstract
Rauvolfia serpentina is an endangered medicinal plant which is used to treat various diseases. Shoot induction and multiplication was achieved from nodal, apical and leaf explants. MS media supplemented with IAA and BAP was found suitable for shoot induction and multiplication. The apical portions of shoot segment gave good results in multiplication. The regenerated shoot when subcultured to same medium shown better proliferation.
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References
- Bhojwani, S.S. (eds.), 1990. Plant Tissue Culture: Applications and Limitations. Elsevier, Amsterdam.
- Bhuya, S.A., Wankhadt, S., G. Paturde, J.T. T and Khode, P.P., 2000. Seed Germination studies in sarpagandha (R. serpentina Benth) Research on crops 1 (2), 189-191.
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References
Bhojwani, S.S. (eds.), 1990. Plant Tissue Culture: Applications and Limitations. Elsevier, Amsterdam.
Bhuya, S.A., Wankhadt, S., G. Paturde, J.T. T and Khode, P.P., 2000. Seed Germination studies in sarpagandha (R. serpentina Benth) Research on crops 1 (2), 189-191.
George, E.F. and Sherrington, P.D., 1984. Plant propagatioan by tissue culture, Hand Book and Directory of commercial Laboratories, Exegetics Ltd., Eversely, Basingstoke, Hants R.G. 27, England.
Kataria, V, Vyas, J.N. and Shekhawat, N., 2005. Cloning of R.serpentina- An endangered Medicinal plant. Journal of sustainable Forestry ;( 2005) vol,-20; Issue-1. DOI: https://doi.org/10.1300/J091v20n01_04
Kirillova, N.V. and Komov, V.P., 2002. Effect of phytohormones on protein synthesizing ability of Rauwolfia serpentine benth. Tissue culture.Prikl Biokhim Microbiol 38(1); 53-6. DOI: https://doi.org/10.1023/A:1013248521076
Sarkar, K.P., Islam, A., Islam, R, Hoque, A. and Jorder, O.I., 1996. In vitro propagation of Rauwolfia serpentine through Tissue culture.Plant Med. 62(4)358-9. DOI: https://doi.org/10.1055/s-2006-957903