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Abstract
Earthworms are commonly known as farmer’s friend. Various previous studies have confirmed the anti-inflammatory, analgesic, antipyretic and anticancer effects of earthworm extract. In the present study selected species of earthworm was used to study the anticoagulative activity of earthworm extract by APTT test. The study involves the extraction and isolation of anticoagulant from earthworm which was found to be in the form of DNA. In order to uncover the biochemical nature of this molecule, the anticoagulant was processed with various hydrolases such as Proteinase-K, Dnase, Rnase and lysozyme. Simultaneously APTT test and agarose gel electrophoresis were performed to confirm the results. Standard herring sperm DNA and yeast RNA were also used to compare the anticoagulative activities with that of anticoagulant purified from earthworm. Individual components of nucleotide were also checked which might be responsible for the anticoagulative capability.
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Copyright (c) 2011 Environment Conservation Journal
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References
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References
Davie E.W., Fujikawa K. and Kisiel W., 1991 The coagulation cascade: initiation, maintenance and regulation. Biochemistry.30, 10363. DOI: https://doi.org/10.1021/bi00107a001
Ehrlich H.J., Keijer J., Preissner K.T., Gebbink, R.K. and Pannekoek, H., 1991 Inhibition of blood coagulation cascade. Biochemistry.30, 1021. DOI: https://doi.org/10.1021/bi00218a020
Kim, Y.S, Kim, J.E, Byun, Chang, H.S., 1995 Regulation of NAD+ glycohydrolase activity by ADP ribosylation. J. Biochem. Mol. Biol.28, 398.
Leipner, C., Tuckova, L., Rejnek , J. and Langer, J., 1993 Serine proteases in coelomic fluid of annelids Eisenia fetida and Lumbricus terrestris. Comp. Biochem.Physiol.105B, 670. DOI: https://doi.org/10.1016/0305-0491(93)90099-Q
Mann, K. G., Nesheim, M.E., Church, W.R. and Krishnaswamy, S., 1990 Surface dependent reactions of the vitamin K- dependent enzyme complexes. Blood.76, 1. DOI: https://doi.org/10.1182/blood.V76.1.1.bloodjournal7611
Mathur, A., Verma, S.K., Bhat, R., Singh, S.K., Prakash, A., Prasad, G.B.K.S. and Dua, V.K., 2010a Antimicrobial activity of earthworm extracts. Journal of Chemical and Pharmaceutical Research. 2, 4, 364-370.
Mathur, A., Verma, S.K., Singh, S.K., Prakash, A., Prasad G.B.K.S. and Dua, V.K., 2010b. Anti-inflammatory activity of earthworm extracts. International Journal of Pharmaceutical Research. 2,1 (In press).
Mathur, A., Mathur, D. and Dua, V.K., 2010. Pharmaceutical Significance of earthworm, Eudrilus eugeniae, Lambert Academic Publishing, Germany.
Nagasawa, H., Sawaki, K., Fuji, Y., Kobayashi, M., Segawa, T., Suzuki, R. and Inatomi, H., 1991 Biology of lysenin a protein in the coelomic fluid of earthworms. Anticancer Res.11, 1061.
Nesheim, M.E., 1983 Various mechanisms of blood coagulation J.Biol.Chem. 258:14708. DOI: https://doi.org/10.1016/S0021-9258(17)43920-2
Noda, N., Tsunefuka, S., Tanaka, R. and Miyahara, K., 1996 Effect of an earthworm, Lumbricus rubellus. Chem.Pharm.Bull. 40, 2756. DOI: https://doi.org/10.1248/cpb.40.2756
Wang, J.D., Narui, T., Kurata, H., Takeuchi, K., Hashimoto, T. and Okuyama, T., 1989 Fibrinolytic activity of the earthworm extract.Chem.Pharm.Bull.37, 2236. DOI: https://doi.org/10.1248/cpb.37.2236
Woo., 1996 Mechanism of Blood coagulation. J. Biochem. Mol. Biol., 29,500.