Main Article Content

Abstract

Cassia fistula belongs to family leguminosae. It is a medium sized tree and its different parts are used in Ayurvedic medicine as well as in home remedies for common ailments. The plant is easily available in Haridwar region. The phytoconstituents of a same plant vary from region to region. In the present study bark of Cassia fistula is used. The material was collected (in Haridwar region, India), dried in shade; powdered and extracted successively with different solvents in an increasing order of polarity. Phytochemical investigation was performed using different identification tests. The different extracts were also screened for antimicrobial activity. For which both Gram positive and Gram negative bacterial strain were selected. Antimicrobial test was performed by agar well diffusion method. All the tests were performed in a triplicate. The different phytoconstituents present in the bark extract are responsible for such an appreciable activity against selected pathogens.

Keywords

Cassia fistula successive extraction phytochemical investigation antimicrobial screening

Article Details

How to Cite
Shukla, R. K., Porval , A., Shukla , A., & Painuly, D. (2012). Preliminary investigation and antimicrobial screening of successive extracts of phytoconstituents from Cassia fistula of Haridwar region, India. Environment Conservation Journal, 13(1&2), 57–62. https://doi.org/10.36953/ECJ.2012.131210

References

  1. Adeniyi, .BA,, Odelola, H,A. and Oso, B.A. ,1996. Antimicrobial potential of Diospyros m.esiliformis (Ebenaceae) Afr. J. Med. Sci. 255: 221-224.
  2. Cosa, P., Vlietink, A.J., Berghe, D.V. and Maes, L., 2006. J. Ethnopharmacol, 106: 290-302. DOI: https://doi.org/10.1016/j.jep.2006.04.003
  3. Cowan, M.M. 1999. Plant products as antimicrobialagents, Clinical microbiology reviews., 12:564-82. DOI: https://doi.org/10.1128/CMR.12.4.564
  4. Dahanukar, S.A., Kulkarni, R.A., Rege, N.N. 2000 Pharmacology of Medicinal Plants and Natural Products, Indian J Pharmacology, 32:81- 118.
  5. Duraipandiyan, V. and Ignacimuthu, S., 2007. J. Ethnopharmacol; 112 :90–594. DOI: https://doi.org/10.1016/j.jep.2007.04.008
  6. Harborne, J.B., 1984. A Guide to Modern Techniques of Plant Analysis. Chapman and Hall, London, 4–80.
  7. Hatano, T., Uebayashi, H., Ito, H., Shiota, S., Tsuchiya, T. and Yoshida, T. 1999. Phenolic constituents of Cassia seeds and antibacterial effect of some naphthalenes and antraquinones on methicillin-resistant Staphylococcus aureus., Chern. Ph atm. Bull, 47(8):1121-1127. DOI: https://doi.org/10.1248/cpb.47.1121
  8. Kokate, C.K., Purohit, A.P and, Gokhale, S.B., 2006. Pharmacognosy. Nirali prakashan, edt. 35: 593-597. DOI: https://doi.org/10.1016/S0755-4982(06)74646-2
  9. Nayan, R., Bhalodia, V. J., Shukla, R. N., Acharya, D. P. and Rajani.2011. Antimicrobial screening of seed extracts of cassia fistula linn. International Journal of Advances in Pharmacy and Nanotechnology, 1 (2): 31 – 35 DOI: https://doi.org/10.5530/ax.2011.1.11
  10. Palombo, E.A. and Semple, S.J., 2002. Antibacterial activity of Australian plant extracts against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE). J Basic Microbiol, 42(6): 444-8. DOI: https://doi.org/10.1002/1521-4028(200212)42:6<444::AID-JOBM444>3.0.CO;2-8
  11. Runyoro, D., Matee, M., Olipa, N., Joseph, C. and Mbwambo, H., (2006). Screening of Tanzanian medicinal plants for anti‐Candida activity, BMC Complement Altern Med., 6(11). DOI: https://doi.org/10.1186/1472-6882-6-11
  12. Shahidi, B.H. 2004. Evaluation of antimicrobial properties of Iranian medicinal plants against Micrococcusluteus, Serratia marcescens, Klebsiella pneumonia and Bordetella bronchoseptica, Asian J Plant Sci., 3:82-86. DOI: https://doi.org/10.3923/ajps.2004.82.86
  13. Stephen, H. Brown Horiculture agent “Cassia fistula” University of Florida.
  14. World Health Organization (WHO) 1978. The promotion and developmentof traditional medicine. Technical report series :622.