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August 28, 2022
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February 11, 2023
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Abstract

Thyroid hormones are known to regulate the basal metabolism rate of an organism. They are also known to regulate the seasonal reproduction of long-day breeding vertebrates in response to thyrotropin induced deiodinase enzymes switching in the brain. The current study attempted to investigate the effect of intraperitoneal administration of thyroxine (T4) and tri-iodothyronine (T3) hormones at various doses on gonadal recrudescence, plasma estradiol-17β and quantitative expression analysis of genes encoding for gonadotropin, thyrotropin, and deiodinases. The estradiol-17β levels were not affected by either thyroid hormone; however, the gonado-somatic index (GSI) and ovarian histology were varying. The gonadotropin releasing hormone 2 (gnrh2) and follicle stimulating hormone-β subunit (fsh-b) gene expressions correspond to the fish GSI and ovarian histology. The gene expressions show that T4 inhibits the expression of thyroid stimulating hormone-β subunit (tsh-b) and type 3 deiodinase (dio3), though it enhances the expression of type 2 deiodinase (dio2). T3, on the other hand, inhibits tsh-b and dio2 expression while increasing dio3 expression. In summary, the T4 appears to regulate gonadal recrudescence in Heteropneustes fossilis in a dose-dependent manner, whereas the T3 appears to have no effect on gonadal activity.

Keywords

Deiodinase Heteropneustes fossilis Reproduction Thyrotropin Thyroid hormones

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Pant, D. R., & Kumari, P. (2023). Effect of exogenously administered thyroid hormones on gonadotropin, thyrotropin and deiodinases encoding genes in the catfish, Heteropneustes fossilis (Bloch). Environment Conservation Journal, 24(1), 261–266. https://doi.org/10.36953/ECJ.17222519
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