Acute high dose Of L-Lysine amino acid leads nephrotoxicity and hepatotoxicity in fresh water fish Clarias batrachus

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B.S. Dhami

Abstract

The present investigation was carried out to find out the effects of high dose of L-lysine on the fish and also an attempt has been made to predict the impact on human health. L-lysine is an essential amino acid which is used in medical practices as a growth promoter of bones especially in infants. Important work on metabolism and functions of amino acids in human health and major diseases has been reported by many workers and evaluated the key role of various amino acids in response to infections. Lysine is not synthesized by body; therefore, it must be taken either by diet or supplementation. Lysine first of all isolated from casein in 1889 and introduced as Lysine hydrochloride in 1955. The recommended dose of lysine is 12mg/kg body weight. Side effects of Lysine in large doses i.e. 10-30gm/day may cause abdominal cramps and diarrhea but renal and hepatic toxicity is not reported in normal person. Amino acids are used to treat end stage renal failure and also in liver failure. Recently lysine is used to treat herpes and as a supplement for diabetic people. In present study the effect of acute high dose 2mg/100gm body weight of L-lysine in fish shows toxic effects on kidney and liver which is an alarming indication towards use of high dose of Lysine in man. In kidney it caused glomerular dilation of the capillaries, glomerular hemorrhages and cloudy swelling of renal tubules. In liver it leads swelling of hepatocytes, vacuolization, fatty degeneration and central vein dilation. These histopathological changes are supported by marked rise in level of blood urea, serum creatinine, blood sugar and serum cholesterol.

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How to Cite
Dhami, B. . (2020). Acute high dose Of L-Lysine amino acid leads nephrotoxicity and hepatotoxicity in fresh water fish Clarias batrachus. Environment Conservation Journal, 21(3), 143-148. https://doi.org/10.36953/ECJ.2020.21317

References

  1. Asanuma, K., Adochi, K., Sugimoto, T. and Chiba, S. 2006. Effects of lysine- induced acute renal failure in dogs. Journal of Toxicological Sciences, 87-98.
  2. Bali and Singh, B.1994. Protective role of L-lysine amino acid in toxicity of Chromium in fish. Biosphere, 6(1): 19-24.
  3. Charlton, M. 2006. Branched- chain amino acid enriched supplements as therapy for liver diseases. Journal of Nutrition,136(1 suppl) : 295S- 8S.
  4. Crim, M. C. and Munro, H. N. 1977. Protein and amino acid requirements & metabolism in relation to defined formula diets: In defined formula diets for medical purposes, ed: M.E. Shils pp. 5-15, American Medical Association, Chicago, IL.
  5. Denis, F., Jing, C., Wei, C., Liliana, G., Hwang, S. J., Kamyar, K., Kopple, Z. J. D., Mitch, W. E. Giorgina, P., Vladimir, T. and Philippe, C. 2016. Adherence to keto acids/ essential amino acids- supplemented low protein diets and new indications for patients with chronic kidney diseases.
  6. Dhami, B. S. 2012. Regenerative effect of L-lysine amino acid on gastric mucosa after chronic chromium toxicity. National seminar on changing scenario in life sciences for future challenges.
  7. Holecek, M. 2010. Three targets of branched- chain amino acid supplementation in the treatment of liver disease. Nutrition, 26(5): 482-90.
  8. John, F. B. 2018. Lysulin a new supplement for Nutritional support for People with Diabetes and Pre- diabetes (those at risk of developing diabetes). Diabetes Management, 38-40.
  9. Jungers, P. and Chauveau, P. 1988. Amino acids and keto acids in the treatment of chronic renal failure. Blood Purification, 6(5): 299-314.
  10. Lee, Da- Young and Kim, Eun-Hee 2019. Therapeutic Effects of Amino Acids in Liver Diseases, Current Studies and Future Perspectives. Journal of Cancer Prevention, 24(2): 72-78.
  11. Malis, C. D., Recusen, L. C., Solez, K. and Whelton, A. 1984. Nephrotoxicity of lysine and of a single dose of aminoglycoside in rats given lysine. Journal of Laboratory and Clinical Medicine, 103: 660-676.
  12. Meenu, S., Muralidhar, R, Shivansh, P., Sowjanya, B., Mahalakshmi, K., Dutt, K. R. and Ramesh, M. 2011. Medicinal uses of L-Lysine: Past and Future. International Journal of Research in Pharmaceutical science (IJRPS), 2(4): 637-642.
  13. Munro, H. N. 1982. Metabolic integration of organs in health and diseases. J PEN.
  14. Muto, Y., Sato, S., Watanabe, A., Moriwaki, H., Suzuki, K., Kato, A., Kato, M., Nakamura, T. and Nishiguchi, S. 2005. Effects of oral branched- chain amino acid granules on event- free survival in patients with liver cirrhosis. Clinical Gastroenterology and Hepatology, 3: 705-713.
  15. Park, J. G., Tak, W. Y. and Lee, W. K. 2017. Effects of branched chain amino acids (BCAAs) on the progression of advanced liver disease. Medicine (Baltimore), 96(24):e6580.
  16. Recusen, L. C., Finn, W. F., Whelton, A. and Solez, K. 1985.Mechanism of lysine- induced acute renal failure in rats. Kidney International, 27: 517-522.
  17. Samyuktha, P. and Francois, V. 2019. Dietary Amino Acids Affect The Rate of Chronic Kidney Disease Progression in Rats. FASEB, 33.S1.
  18. Solez, K. 1983. Pathogenesis of acute renal failure. International review of experimental pathology, 24: 277-333.
  19. Steven, G., Munz, K.and Ulbricht, C. 2007. A Review of Dietary Supplement – Induced Renal Dysfunction. Clinical Journal of the American Society of Nephrology, 2: 757-765.
  20. Tahira, S. and Khan, N. 2015. Nutrition as a part of therapy in treatment of liver cirrhosis. Journal of Nutrition & Food Sciences, S11-004.
  21. Venthan, J. M. and Sanketh, R. 2017. Lysine for Herpes Simplex Prophylaxis: A Review of the Evidence. Integrative medicine (Encinitas, Calif.) 16(3): 42-46.